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Hong Tang Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Xiaomei Jiang Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Yu Hua Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Heyue Li Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Chunlan Zhu Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Xiaobai Hao Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Minhui Yi Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Linxia Li Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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-phosphate dehydrogenase) (1:5000, Abcam, ab9485). The membranes were washed with TBST (TBS with Tween-20) for three times after incubation with primary antibodies for 2 h. Thereafter, membranes were incubated with goat anti-rabbit IgG H&L (HRP) (1:5000, ab

Article Type:
Research Article
Collections:
Reproduction
DOI:
https://doi.org/10.1530/EC-22-0459
Volume/Issue:
Volume 12: Issue 4
Open access
Li Qian Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China
Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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Yuxiao Zhu Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China

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Yan Luo Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China

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Mu Zhang Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China

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Liping Yu Barbara Davis Center for Childhood Diabetes, University of Colorado School of Medicine, Aurora, Colorado, USA

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Yu Liu Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, Jiangsu, China

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Tao Yang Department of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

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-risk autoantibodies and greatly improve sensitivity and disease specificity. Moreover, ECL assays, which capture all of the immunoglobulin classes (IgG, IgM, IgA, and IgE), are more sensitive than ELISA, which captures only IgG. More importantly, previous studies have

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-22-0325
Volume/Issue:
Volume 11: Issue 12
Open access
A Daniel Bird Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia

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Spencer Greatorex Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia

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David Reser Department of Physiology, Monash University, Melbourne, Victoria, Australia

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Gareth G Lavery Institute of Metabolism and Systems Research, 2nd Floor IBR Tower, University of Birmingham, Birmingham, UK
Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK

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Timothy J Cole Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia

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immunostained with primary antibodies (rabbit anti-GRP78 and a mouse anti-HSD1L monoclonal antibody produced in the laboratory to a hHSD1L peptide), then washed and detected with secondary antibodies (anti-rabbit IgG alexa-488 and anti-mouse IgG2a alexa-555

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0119
Volume/Issue:
Volume 6: Issue 7
Open access
Doron Weinstein
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Rive Sarfstein
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Zvi Laron Department of Human Molecular Genetics and Biochemistry, Endocrinology and Diabetes Research Unit, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel

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Haim Werner
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antibodies were HRP-conjugated goat anti-rabbit IgG (1:50 000) and donkey anti-mouse IgG (1:25 000; Jackson ImmunoResearch Laboratories, West Grove, PA, USA). Proteins were detected using the SuperSignal West PicoChemiluminescent Substrate (Pierce, Rockford

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-13-0086
Volume/Issue:
Volume 3: Issue 1
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Qing Zhu Department of Endocrinology, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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Jianbin Su Department of Endocrinology, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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Xueqin Wang Department of Endocrinology, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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Mengjie Tang Department of Endocrinology, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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Yingying Gao Department of Rheumatology, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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Dongmei Zhang Clinical Medicine Research Center, Nantong City No 1 People’s Hospital and Second Affiliated Hospital of Nantong University, Jiangsu, China

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.25(0.40–79.8) 0.10(0.10–0.25) < 0.001 Thyroid VolT (mL) 16.9(12.5–22.5) n – IgG (g/L) 14.1 ± 3.88 13.3 ± 2.19 0.562 IgM (g/L) 1.19 ± 0.57 1.36 ± 0.58 0.171 IgA (g/L) 2.02(1.61–2.69) 2.16(1.64–2.95) 0.311 C3

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0162
Volume/Issue:
Volume 9: Issue 7
Open access
Ming Zhu Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Bingxin Xu Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Meng Wang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Shangyun Liu Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Yue Zhang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Chao Zhang Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China

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Interaction of MRAP2 proteins with slMCa or slMCb. Co-IP of slMCa or slMCb with four types of MRAP2 proteins: (A and B) slMRAP2, (C and D) esMRAP2-C, (E and F) zMRAP2a-C and (G and H). *IgG heavy chain. (I) Schematic diagram of four MRAP2 plasmids. N terminus

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0019
Volume/Issue:
Volume 8: Issue 4
Open access
I Savchuk Department of Women’s and Children’s Health, Pediatric Endocrinology Unit, Karolinska Institute & University Hospital, Stockholm, Sweden

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M L Morvan LUNAM Université, École Nationale Vétérinaire, Agroalimentaire et de l’Alimentation, Nantes-Atlantique (Oniris), Laboratoire d’Étude des Résidus et Contaminants dans les Aliments (LABERCA), USC INRA 1329, Nantes, France

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J P Antignac LUNAM Université, École Nationale Vétérinaire, Agroalimentaire et de l’Alimentation, Nantes-Atlantique (Oniris), Laboratoire d’Étude des Résidus et Contaminants dans les Aliments (LABERCA), USC INRA 1329, Nantes, France

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K Gemzell-Danielsson Department of Obstetrics and Gynecology, Karolinska Institute & University Hospital, Stockholm, Sweden

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B Le Bizec LUNAM Université, École Nationale Vétérinaire, Agroalimentaire et de l’Alimentation, Nantes-Atlantique (Oniris), Laboratoire d’Étude des Résidus et Contaminants dans les Aliments (LABERCA), USC INRA 1329, Nantes, France

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O Söder Department of Women’s and Children’s Health, Pediatric Endocrinology Unit, Karolinska Institute & University Hospital, Stockholm, Sweden

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K Svechnikov Department of Women’s and Children’s Health, Pediatric Endocrinology Unit, Karolinska Institute & University Hospital, Stockholm, Sweden

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5αR1 ( Table 2 ) or unspecific IgGs (for negative control) dissolved in 3% goat serum in PBS overnight at 4°C. After washing with PBS and 0.01% Tween 20, the slides were incubated with biotinylated secondary antibody (ab64256, Abcam), and then with

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0085
Volume/Issue:
Volume 6: Issue 6
Open access
Estíbaliz Castillero
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Ana Isabel Martín
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Maria Paz Nieto-Bona Department of Physiology, Department of Histology, Faculty of Medicine, Complutense University of Madrid, 28040 Madrid, Spain

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Carmen Fernández-Galaz
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María López-Menduiña
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María Ángeles Villanúa
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Asunción López-Calderón
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–Aldrich) with stripping of membranes before each new antibody. Membranes were then incubated for 90 min in the appropriate secondary antibody conjugated to HRP (antimouse IgG, Amersham Biosciences; antirabbit IgG, Bio-Rad), and peroxidase activity was detected

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-12-0003
Volume/Issue:
Volume 1: Issue 1
Open access
Elvira C Arellanes-Licea Neurobiología Celular y Molecular, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, México

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José Ávila-Mendoza Neurobiología Celular y Molecular, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, México

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Elizabeth C Ramírez-Martínez Neurobiología Celular y Molecular, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, México

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Eugenia Ramos Neurobiología Celular y Molecular, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, México

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Nancy Uribe-González Neurobiología Celular y Molecular, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, México

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Carlos Arámburo Neurobiología Celular y Molecular, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, México

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Teresa Morales Neurobiología Celular y Molecular, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, México

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Maricela Luna Neurobiología Celular y Molecular, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, México

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antibody was detected by using a Cy3-goat anti-rabbit IgG secondary antibody (Abcam; 1:5000), the labeled sections were placed with DAPI (Sigma, D-9542, at a final concentration of 0.1 µg/mL) stocks solution and washed. The sections were mounted, dehydrated

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0380
Volume/Issue:
Volume 7: Issue 2
Open access
Thorben Hoffmann Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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Yousef Ashraf Tawfik Morcos Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany
Center for Biochemistry, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany

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Ruth Janoschek Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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Eva-Maria Turnwald Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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Antje Gerken Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany
Center for Biochemistry, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany

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Annette Müller Center for Pediatric Pathology at the University Hospital Cologne, Cologne, Germany

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Gerhard Sengle Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany
Center for Biochemistry, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany
Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany
Cologne Center for Musculoskeletal Biomechanics (CCMB), Cologne, Germany

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Jörg Dötsch Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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Sarah Appel Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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Eva Hucklenbruch-Rother Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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secondary antibody (polyclonal rabbit anti-mouse IgG-HRP conjugate P026002-2 (Agilent Dako) was added 1:2000 in 2.5% milk/PBS for 30 min at RT. The plate was washed again and 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific) was added and

Article Type:
Research Article
Collections:
Bone and Mineral Metabolism
DOI:
https://doi.org/10.1530/EC-22-0069
Volume/Issue:
Volume 11: Issue 3
Open access