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Marco Marino Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy
Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Valentina Cirello Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy
Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Valentina Gnarini Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Carla Colombo Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy
Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Elisa Pignatti Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy
Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Livio Casarini Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy
Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Chiara Diazzi Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Vincenzo Rochira Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy
Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Katia Cioni Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Bruno Madeo Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Cesare Carani Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy
Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Manuela Simoni Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy
Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy
Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Laura Fugazzola Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy
Unit and Chair of Endocrinology and Metabolism, Center for Genomic Research, Department of Clinical Sciences and Community Health, Endocrine Unit, Azienda USL of Modena, NOCSAE, Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Via Pietro Giardini 1355, 41126 Modena, Italy

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Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy, with a steadily increasing incidence in the last few decades worldwide. The predisposition to developing this carcinoma by the heterozygous state of rs2910164 within the precursor of the miR-146a has been reported, but recently not confirmed. Interestingly, on the same chromosome, almost 50 kb separate the pre-miR-146a from the pituitary tumor-transforming gene 1 (PTTG1), a proto-oncogene involved in several tumors, including thyroid cancers. In this study, we analyzed, using a case–control design, the genetic association between PTC and the genomic region encompassing pre-miR-146a rs2910164 and PTTG1 rs1862391 and rs2910202. We enrolled 307 affected patients and 206 healthy controls. The possible presence of thyroid nodules in controls was excluded by ultrasonography. All the cases were submitted to single-nucleotide polymorphism (SNP) genotyping of pre-miR-146a and PTTG1, and risk association analyses were carried out. The genotypic and allelic frequencies of pre-miR-146a rs2910164 were not statistically different in the patients and controls, and this SNP was not in linkage disequilibrium with the investigated PTTG1 SNPs. Consistently, meta-analyses, the first including all the affected cases published to date, did not confirm the previously reported association of the heterozygous CG genotype with PTC. The PTTG1 SNPs exhibited the same allelic frequency in the patients and controls and were not associated with the disease. In conclusion, in a well-selected Italian population, neither pre-miR-146a rs2910164 nor PTTG1 rs1862391 and rs2910202 were found to be associated with the risk of developing PTC.

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-13-0066
Volume/Issue:
Volume 2: Issue 4
Open access
Xue-Lian Zhang Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, China

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Xinyi Zhao Department of Physiology, School of Medicine, Jinan University, Guangzhou, China

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Yong Wu Department of Physiology, School of Medicine, Jinan University, Guangzhou, China
Department of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic University, Hong Kong, China

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Wen-qing Huang Department of Transfusion Medicine, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong, China

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Jun-jiang Chen Department of Physiology, School of Medicine, Jinan University, Guangzhou, China
Department of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic University, Hong Kong, China

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Peijie Hu Department of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic University, Hong Kong, China

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Wei Liu Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, China

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Yi-Wen Chen Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, China

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Jin Hao Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, China

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Rong-Rong Xie Department of Endocrinology, Beijing Tongren Hospital, Capital Medical University, Beijing, China

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Hsiao Chang Chan Epithelial Cell Biology Research Center, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China

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Ye Chun Ruan Department of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic University, Hong Kong, China

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Hui Chen Cell-Gene Therapy Translational Medicine Research Center, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China

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Jinghui Guo Department of Physiology, School of Medicine, Jinan University, Guangzhou, China

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(Catalog# CRL11605™, ATCC) and grown in RPMI1640 medium (Catalog# 11875101, Thermo Fisher Scientific) with 10% fetal bovine serum (Catalog# 16000044, Thermo Fisher Scientific), 100 IU/mL penicillin and 100 µg/mL streptomycin. ELISA measurement of

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-21-0357
Volume/Issue:
Volume 11: Issue 1
Open access
Zhiwei Zhang Department of Obstetrics and Gynecology, Liaocheng People’s Hospital, Liaocheng, Shandong, China

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Hui Zhao Department of Obstetrics and Gynecology, Liaocheng People’s Hospital, Liaocheng, Shandong, China

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Aixia Wang Department of Obstetrics and Gynecology, Liaocheng People’s Hospital, Liaocheng, Shandong, China

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, glucose levels were analyzed using a glucose meter (Roche Diagnostics). An ultra-sensitive mouse insulin ELISA kit (ALP CO Diagnostics) was used to measure insulin levels. Pathological investigation First, animals were anesthetized by ether, then

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0466
Volume/Issue:
Volume 10: Issue 1
Open access
V Kenan Çelik Department of Biochemistry, Cumhuriyet University School of Medicine, Sivas, Turkey

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Sercan Kapancık Department of Biochemistry, Cumhuriyet University School of Medicine, Sivas, Turkey

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Turgut Kaçan Department of Medical Oncology, High Specialized Education and Research Hospital, Bursa, Turkey

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Selen Baloglu Kaçan Department of Internal Medicine, Afyonkarahisar State Hospital, Afyon, Turkey

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Serkan Kapancık Department of Biochemistry, Cumhuriyet University School of Medicine, Sivas, Turkey

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Hasan Kılıçgün Department of Nutrition and Dietetics, Health Science Faculty, University of Erzincan, Erzincan, Turkey

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method defined by Chinard and was reported as μmol/mL ( 26 ). Arginine decarboxylase, ornithine decarboxylase and agmatinase levels were determined by ELISA kit according to the manufacturer’s protocol (Cusabio Biotech, Wuhan, China) and expressed as pg

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0137
Volume/Issue:
Volume 6: Issue 8
Open access
Thorben Hoffmann Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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Yousef Ashraf Tawfik Morcos Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany
Center for Biochemistry, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany

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Ruth Janoschek Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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Eva-Maria Turnwald Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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Antje Gerken Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany
Center for Biochemistry, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany

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Annette Müller Center for Pediatric Pathology at the University Hospital Cologne, Cologne, Germany

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Gerhard Sengle Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany
Center for Biochemistry, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany
Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany
Cologne Center for Musculoskeletal Biomechanics (CCMB), Cologne, Germany

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Jörg Dötsch Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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Sarah Appel Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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Eva Hucklenbruch-Rother Department of Pediatrics, and Adolescent Medicine, Faculty of Medicine and University Hospital of Cologne, University of Cologne, Cologne, Germany

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umbilical blood ( 14 ). Since there is an urgent need for a reliable and sensitive method to determine plasma asprosin levels ( 15 ), our laboratory took the initiative to develop a functional and dependable asprosin ELISA ( 16 ). Here, we set out to test

Article Type:
Research Article
Collections:
Bone and Mineral Metabolism
DOI:
https://doi.org/10.1530/EC-22-0069
Volume/Issue:
Volume 11: Issue 3
Open access
Marra Jai Aghajani Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia

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Tara Laurine Roberts Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
South West Sydney Clinical School, UNSW Sydney, Sydney, Australia

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Tao Yang School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
Saint Vincent’s Clinical School, UNSW Sydney, Sydney, Australia
SydPath, Saint Vincent’s Hospital, Sydney, Australia

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Charles Eugenio McCafferty Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia

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Nicole J Caixeiro Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
Centre for Oncology Education and Research Translation (CONCERT), Liverpool, New South Wales, Australia

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Paul DeSouza Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
South West Sydney Clinical School, UNSW Sydney, Sydney, Australia

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Navin Niles Ingham Institute for Applied Medical Research, Liverpool, New South Wales, Australia
School of Medicine, Western Sydney University, Campbelltown, New South Wales, Australia
Department of Head & Neck Surgery, Liverpool Hospital, Liverpool, New South Wales, Australia
Department of Clinical Medicine, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, Australia

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healthy controls were collected in the same manner. Healthy controls were defined as volunteers with no active medical conditions. sPD-L1 ELISAs Soluble PD-L1 (sPD-L1) was quantified using a commercially available ELISA (PDCD1LG1 ELISA kit, USCN

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0210
Volume/Issue:
Volume 8: Issue 7
Open access
Aleksandra Krygier Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland

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Ewelina Szczepanek-Parulska Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland

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Dorota Filipowicz Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland

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Marek Ruchała Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland

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. Figure 2 Factors affecting hepcidin level, constituting the exclusion criteria of our study. The level of hepcidin was measured by the Hepcidin 25 (bioactive) hs ELISA, a highly sensitive enzyme immunoassay for the quantitative in vitro

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0017
Volume/Issue:
Volume 9: Issue 3
Open access
Hong Tang Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Xiaomei Jiang Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Yu Hua Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Heyue Li Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Chunlan Zhu Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Xiaobai Hao Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Minhui Yi Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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Linxia Li Departments of Gynaecology and Obstetrics Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China

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< 0.01; ns, not significant. (F–I) The level of Fe 2+ , the content of MDA, the level of ROS, and the content of GSH in NEDD4L-OE GCs was analyzed by ELISA ( n  = 3) * P < 0.05, ** P < 0.01. Multiple modes of programmed cell death are

Article Type:
Research Article
Collections:
Reproduction
DOI:
https://doi.org/10.1530/EC-22-0459
Volume/Issue:
Volume 12: Issue 4
Open access
Xuhua Mao Department of Clinical Laboratory, Yixing People’s Hospital, Yixing, Wuxi, Jiangsu, China

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Hucheng Chen Department of Nuclear Medicine, Nanjing Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu, China

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Junmin Tang Department of Clinical Laboratory, Yixing People’s Hospital, Yixing, Wuxi, Jiangsu, China

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Liangliang Wang Department of Neurology, Yixing People’s Hospital Affiliated to Jiangsu University, Yixing, Wuxi, Jiangsu, China

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Tingting Shu Department of Central Laboratory, Jiangsu Province Official Hospital, Nanjing, Jiangsu, China

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from Promega. Insulin ELISA kits were purchased from Merodia (Uppsala, Sweden). Hepcidin ELISA kits were purchased from Uscn (Wuhan, China). Cells and cell culture The mouse insulinoma cell line, MIN6 was purchased from the American Type Culture

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-16-0115
Volume/Issue:
Volume 6: Issue 3
Open access
Jay Toulany College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, Canada

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Sebastian D Parlee Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA

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Christopher J Sinal Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada

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Kathryn Slayter Canadian Centre for Vaccinology, IWK Health Centre, Nova Scotia Health Authority and Dalhousie University, Halifax, Nova Scotia, Canada

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Shelly McNeil Canadian Centre for Vaccinology, IWK Health Centre, Nova Scotia Health Authority and Dalhousie University, Halifax, Nova Scotia, Canada

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Kerry B Goralski College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, Canada
Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada

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of CMKLR1 receptor activation (expressed as chemerin 157 equivalents) in each sample was interpolated based on its luciferase/β-galactosidase activity. Total chemerin measurements Total chemerin was quantified using a human pan-chemerin ELISA

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-16-0065
Volume/Issue:
Volume 5: Issue 6
Open access