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A Daniel Bird Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia

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Spencer Greatorex Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia

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David Reser Department of Physiology, Monash University, Melbourne, Victoria, Australia

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Gareth G Lavery Institute of Metabolism and Systems Research, 2nd Floor IBR Tower, University of Birmingham, Birmingham, UK
Centre for Endocrinology, Diabetes and Metabolism, Birmingham Health Partners, Birmingham, UK

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Timothy J Cole Department of Biochemistry and Molecular Biology, Monash University, Melbourne, Victoria, Australia

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sheep and human tissue or cell samples were analysed using a Rotor-Gene 3000 PCR (Qiagen), with each biological replicate assayed in triplicate using SYBR Green qPCR SuperMix (Invitrogen). Rps29 and 18S-rRNA levels were used as a normalising RNA control

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0119
Volume/Issue:
Volume 6: Issue 7
Open access
Alice Costantini Department of Molecular Medicine and Surgery and Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden

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Mari H Muurinen Folkhälsan Institute of Genetics, University of Helsinki, Helsinki, Finland
Children’s Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland
Research Program for Clinical and Molecular Metabolism, University of Helsinki, Helsinki, Finland

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Outi Mäkitie Department of Molecular Medicine and Surgery and Center for Molecular Medicine, Karolinska Institutet, Stockholm, Sweden
Folkhälsan Institute of Genetics, University of Helsinki, Helsinki, Finland
Children’s Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland
Research Program for Clinical and Molecular Metabolism, University of Helsinki, Helsinki, Finland
Department of Clinical Genetics, Karolinska University Hospital, Stockholm, Sweden

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-associated variants in different categories of disease located in distinct regulatory elements . BMC Genomics 2015 16 (Supplement 8) S3. ( https://doi.org/10.1186/1471-2164-16-S8-S3 ) 68 Gonorazky HD Naumenko S Ramani AK Nelakuditi V Mashouri P Wang

Article Type:
Review Article
DOI:
https://doi.org/10.1530/EC-21-0083
Volume/Issue:
Volume 10: Issue 5
Open access
Elham Barazeghi Department of Surgical Sciences, Uppsala University, Uppsala University Hospital, Rudbeck Laboratory, Uppsala, Sweden

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Per Hellman Department of Surgical Sciences, Uppsala University, Uppsala University Hospital, Rudbeck Laboratory, Uppsala, Sweden

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Gunnar Westin Department of Surgical Sciences, Uppsala University, Uppsala University Hospital, Rudbeck Laboratory, Uppsala, Sweden

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Peter Stålberg Department of Surgical Sciences, Uppsala University, Uppsala University Hospital, Rudbeck Laboratory, Uppsala, Sweden

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for PTPRM (Hs00267809_m1), GAPDH (Hs02758991_g1) and 18S rRNA (Hs03928990_g1) transcripts. All samples were amplified in triplicates. Puromycin- N -acetyltransferase gene ( pac ) expression analysis was performed on Stratagene Mx3005P real-time PCR

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0279
Volume/Issue:
Volume 8: Issue 8
Open access
Sarmistha Banerjee Department of Biomedical Sciences, University of Pennsylvania, Philadelphia, Pennsylvania, USA

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Allison M Hayes Department of Biomedical Sciences, University of Pennsylvania, Philadelphia, Pennsylvania, USA

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Bernard H Shapiro Department of Biomedical Sciences, University of Pennsylvania, Philadelphia, Pennsylvania, USA

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and purity were determined by UV spectrophotometry (A260/280 > 1.8 and A260/240 > 1.7) and integrity was verified by the intensities of 28S and 18S rRNA bands on a denaturing agarose gel visualized on a FluorChem IS-8800 Imager (Alpha Innotech, San

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0143
Volume/Issue:
Volume 10: Issue 9
Open access
Kylie D Rock Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina, USA

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Brian Horman Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina, USA

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Allison L Phillips Nicholas School of the Environment, Duke University, Durham, North Carolina, USA

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Susan L McRitchie NIH Eastern Regional Comprehensive Metabolomics Res. Core, Univ. of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

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Scott Watson NIH Eastern Regional Comprehensive Metabolomics Res. Core, Univ. of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

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Jocelin Deese-Spruill NIH Eastern Regional Comprehensive Metabolomics Res. Core, Univ. of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA

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Dereje Jima Center for Human Health and the Environment, North Carolina State University, Raleigh, North Carolina, USA
Bioinformatics Research Center, North Carolina State University, Raleigh, North Carolina, USA

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Susan Sumner NIH Eastern Regional Comprehensive Metabolomics Res. Core, Univ. of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
Center for Human Health and the Environment, North Carolina State University, Raleigh, North Carolina, USA

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Heather M Stapleton Nicholas School of the Environment, Duke University, Durham, North Carolina, USA

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Heather B Patisaul Department of Biological Sciences, North Carolina State University, Raleigh, North Carolina, USA
Center for Human Health and the Environment, North Carolina State University, Raleigh, North Carolina, USA

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detailed ( 53 ). Triplicate reactions were run as well as negative controls (no template present) for each TaqMan assay. A house keeping gene (18s rRNA) was used to normalize CT values for differences in starting concentrations of cDNA. Relative changes in

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0373
Volume/Issue:
Volume 7: Issue 2
Open access