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Xiaolei Hu Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China
Department of Endocrinology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

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Fengling Chen Department of Endocrinology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

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summarizes the characterization of IAs, the factors affecting IA development, the clinical significance of IAs and the treatments for EIAS. Characterization of IAs IAs’ representatives are found in all of the Ig classes. Among them, IgG is predominant

Article Type:
Review Article
DOI:
https://doi.org/10.1530/EC-17-0309
Volume/Issue:
Volume 7: Issue 1
Open access
Stefan Schulz
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Anika Mann
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Benjamin Novakhov
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Hugh D Piggins Institute of Pharmacology and Toxicology, Faculty of Life Sciences, Jena University Hospital, Friedrich Schiller University Jena, Drackendorfer Straße 1, D-07747 Jena, Germany

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Amelie Lupp
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 °C. Detection of the primary antibody was performed using a biotinylated goat anti-rabbit IgG followed by an incubation with peroxidase-conjugated avidin (Vector ABC ‘Elite’ Kit, Vector Laboratories, Burlingame, CA, USA). Binding of the primary

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-14-0051
Volume/Issue:
Volume 4: Issue 1
Open access
Jana Ernst Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

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Urszula Grabiec Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

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Kathrin Falk Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

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Faramarz Dehghani Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

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Kristina Schaedlich Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

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Signaling, Boston, USA  Rabbit antibody against human GAPDH a 1:1000 Cell Signaling Secondary antibody  Anti-rabbit-IgG 1:20,000 Vektor laboratories, Burlingame, CA  Anti-mouse-IgG 1:10,000 Vektor laboratories

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0548
Volume/Issue:
Volume 9: Issue 2
Open access
Anne-Sophie C A M Koning Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Philippe C Habets Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Marit Bogaards Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Jan Kroon Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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Hanneke M van Santen Department of Pediatric Endocrinology, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht, The Netherlands
Department of Pediatric Neuro-Oncology, Prinses Máxima Centrum, Utrecht, The Netherlands

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Judith M de Bont Department of Pediatric Neuro-Oncology, Prinses Máxima Centrum, Utrecht, The Netherlands

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Onno C Meijer Division of Endocrinology, Department of Medicine, Leiden University Medical Center, Leiden, The Netherlands
Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

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rabbit monoclonal GR antibody (1:500, D6H2L, Cell Signalling technology) and mouse monoclonal MR antibody (1:500, MR1-18 1D5 and MR 4G5) ( 20 ) in 1% BSA/PBST. After washing, sections were incubated with AlexaFluor-488 labeled goat-anti-rabbit IgG and

Article Type:
Research Article
Collections:
Cardiovascular
DOI:
https://doi.org/10.1530/EC-21-0425
Volume/Issue:
Volume 11: Issue 3
Open access
Yi Jia School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Yanan Yang School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Jing Qu School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Lijun Yin School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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Xiaohui Wang School of Health and Exercise, The Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai, China

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:1000,2438S, Cell Signaling Technology) primary antibodies. The membranes were incubated at room temperature for 1 h with the secondary antibodies (HRP-labeled goat anti-mouse IgG(H+L),1:1000, A0216, Beyotime; goat anti-rabbit IgG(H+L)-HRP antibody, 1

Article Type:
Research Article
Collections:
Metabolic Syndrome and Diabetes
DOI:
https://doi.org/10.1530/EC-23-0484
Volume/Issue:
Volume 13: Issue 4
Open access
Ping Gu Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China

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Yuege Lin Department of Pathology, Nanjing Medical University, Nanjing, China

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Qi Wan Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China

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Dongming Su Department of Pathology, Nanjing Medical University, Nanjing, China

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Qun Shu Department of Obstetrics, Shanghai First Maternity and Infant Health Hospital, School of Medicine, Tongji University, Shanghai, China

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anti-goat/mouse IgG and Alexa Fluor 488 conjugated donkey anti-rabbit/rat IgG (1:400, Invitrogen). The nuclei were stained with 4′,DAPI (Sigma–Aldrich). For immunohistochemical staining, the secondary antibody was HRP-conjugated goat anti

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0043
Volume/Issue:
Volume 10: Issue 7
Open access
Ingeborg Brønstad Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway

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Lars Breivik Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway

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Paal Methlie Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway
Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway

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Anette S B Wolff Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway

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Eirik Bratland Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway

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Ingrid Nermoen Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway

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Kristian Løvås Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway
Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway

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Eystein S Husebye Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway
Department of Clinical Science, Department of Medicine, Division of Medicine, University of Bergen, 5021 Bergen, Norway

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against 21OH (c17, goat polyclonal IgG, Santa Cruz Biotechnology) and alkaline phosphatase-conjugated donkey anti-goat IgG secondary antibody (Santa Cruz Biotechnology). Relative quantitation of 21OH from in vitro expression ELISA plates (MaxiSorp, Nunc

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-14-0032
Volume/Issue:
Volume 3: Issue 2
Open access
Melinda Kertész Department of Medicine and Nephrology-Diabetes Centre, Medical School, University of Pécs, Pécs, Baranya, Hungary

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Szilárd Kun Department of Medicine and Nephrology-Diabetes Centre, Medical School, University of Pécs, Pécs, Baranya, Hungary

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Eszter Sélley Department of Medicine and Nephrology-Diabetes Centre, Medical School, University of Pécs, Pécs, Baranya, Hungary

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Zsuzsanna Nagy Department of Medicine and Nephrology-Diabetes Centre, Medical School, University of Pécs, Pécs, Baranya, Hungary

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Tamás Kőszegi Department of Laboratory Medicine, Medical School, University of Pécs, Pécs, Baranya, Hungary

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István Wittmann Department of Medicine and Nephrology-Diabetes Centre, Medical School, University of Pécs, Pécs, Baranya, Hungary

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% methanol to PVDF membranes (Amersham-Biotech) for 90 min at 250 mA. Membranes were washed with TBS-T 0.1% and incubated in peroxidase-conjugated IgG secondary antibody diluted in TBS-T with 5% BSA to 1:2000 with anti-rabbit polyclonal anti-phospho-(Ser473

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0218
Volume/Issue:
Volume 10: Issue 7
Open access
Hichem Bouguerra Université Tunis El-Manar, Faculté des Sciences de Tunis, Laboratoire de Génétique, Immunologie et pathologies Humaines, Tunis, Tunisie
Université Côte d'Azur, INSERM, C3M, Team Cellular and Molecular Physiopathology of Obesity and Diabetes, Nice, France

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Gorrab Amal Université Tunis El-Manar, Faculté des Sciences de Tunis, Laboratoire de Génétique, Immunologie et pathologies Humaines, Tunis, Tunisie

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Stephan Clavel Université Côte d'Azur, INSERM, C3M, Team Cellular and Molecular Physiopathology of Obesity and Diabetes, Nice, France

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Hamouda Boussen Département d’Oncologie Médicale, Hôpital Abderrahman Mami, Ariana, Tunisia

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Jean-François Louet Université Côte d'Azur, INSERM, C3M, Team Cellular and Molecular Physiopathology of Obesity and Diabetes, Nice, France

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Asma Gati Université Tunis El-Manar, Faculté des Sciences de Tunis, Laboratoire de Génétique, Immunologie et pathologies Humaines, Tunis, Tunisie

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IgG conjugated with HRP was used as secondary antibody. Immunodetection was performed using enhanced chemiluminescence (ECL system, Amersham Biosciences Inc.) according to the manufacturer’s instructions. RNA isolation and Real-time RT

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0109
Volume/Issue:
Volume 9: Issue 6
Open access
Qi Che Reproductive Medicine Center, Zhongshan Hospital, Fudan University, Shanghai, China

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Xirong Xiao Department of Obstetrics, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, China

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Jun Xu Reproductive Medicine Center, Zhongshan Hospital, Fudan University, Shanghai, China

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Miao Liu Reproductive Medicine Center, Zhongshan Hospital, Fudan University, Shanghai, China

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Yongning Lu Reproductive Medicine Center, Zhongshan Hospital, Fudan University, Shanghai, China

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Suying Liu Reproductive Medicine Center, Zhongshan Hospital, Fudan University, Shanghai, China

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Xi Dong Reproductive Medicine Center, Zhongshan Hospital, Fudan University, Shanghai, China

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. IL-6 neutralizing antibody was from R&D systems. Anti-total Stat3, anti-phospho Stat3 (p-Stat3), anti-Bcl-2, anti-myeloid cell leukemia-1 (Mcl-1), anti-CyclinD1, anti-matrix metalloproteinases 2 (MMP2) antibodies and rabbit IgG were from Epitomics (CA

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0258
Volume/Issue:
Volume 8: Issue 7
Open access