L Ahlkvist
L Ahlkvist
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K Brown
K Brown
Department of Clinical Sciences, GlaxoSmithKline, Biomedical Center, C11, Lund University, SE 22184 Lund, Sweden
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B Ahrén
B Ahrén
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, Offenburg, Germany). Insulin was analyzed with sandwich immunoassay technique (ELISA) using double MABs against insulin (Mercodia, Uppsala, Sweden). Glucagon was measured by RIA (Millipore, Billerica, MA, USA). Levels of intact GLP1 were determined by
Nicolai Preisler
Nicolai Preisler
Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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Pascal Laforêt
Pascal Laforêt
Centre de Référence de Pathologie Neuromusculaire Paris-Est, Institut de Myologie, GH Pitié-Salpêtrière, Assistance Publique-Hôpitaux de Paris, Paris, France
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Karen Lindhardt Madsen
Karen Lindhardt Madsen
Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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Edith Husu
Edith Husu
Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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Christoffer Rasmus Vissing
Christoffer Rasmus Vissing
Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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Gitte Hedermann
Gitte Hedermann
Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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Henrik Galbo
Henrik Galbo
Department of Inflammation Research, Rigshospitalet, Copenhagen, Denmark
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Christopher Lindberg
Christopher Lindberg
Department of Neurology, Sahlgrenska University Hospital, Gothenburg, Sweden
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John Vissing
John Vissing
Copenhagen Neuromuscular Center, Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
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Chemical Gmbh, Germany). Insulin and catecholamines were measured at rest and after exercise with enzyme-linked immunosorbent assays (Insulin ELISA kit, Dako, Glostrup, Denmark, and 2-CAT plasma ELISA kit, Labor Diagnostica Nord GmbH & Co) and a Modulus II
Kaisa K Ivaska
Kaisa K Ivaska
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Maikki K Heliövaara
Maikki K Heliövaara
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
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Pertti Ebeling
Pertti Ebeling
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
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Marco Bucci
Marco Bucci
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
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Ville Huovinen
Ville Huovinen
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
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Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
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H Kalervo Väänänen
H Kalervo Väänänen
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Pirjo Nuutila
Pirjo Nuutila
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
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Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
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Heikki A Koistinen
Heikki A Koistinen
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
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Department of Cell Biology and Anatomy, Department of Medicine, Turku PET Centre, Department of Radiology, Medical Imaging Centre of Southwest Finland, Department of Endocrinology, Abdominal Center: Endocrinology, Minerva Foundation Institute for Medical Research, Institute of Biomedicine, University of Turku, FI-20520 Turku, Finland
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telopeptides of type I collagen (CTX) and tartrate-resistant acid phosphatase 5b (TRAcP5b) using serum IDS-iSYS CTX-I (CrossLaps) ELISA and BoneTRAP (TRAcP 5b) ELISA (both from IDS Ltd, Boldon, Tyne and Wear, UK). Bone formation was assessed by measuring serum
Nancy J Olsen
Nancy J Olsen
Division of Rheumatology, Division of Endocrinology, Diabetes, and Metabolism, College of Medicine, Milton S Hershey Medical Center, The Pennsylvania State University, Mail Code H044, 500 University Drive, Hershey, Pennsylvania 17033-0850, USA
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Ann L Benko
Ann L Benko
Division of Rheumatology, Division of Endocrinology, Diabetes, and Metabolism, College of Medicine, Milton S Hershey Medical Center, The Pennsylvania State University, Mail Code H044, 500 University Drive, Hershey, Pennsylvania 17033-0850, USA
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William J Kovacs
William J Kovacs
Division of Rheumatology, Division of Endocrinology, Diabetes, and Metabolism, College of Medicine, Milton S Hershey Medical Center, The Pennsylvania State University, Mail Code H044, 500 University Drive, Hershey, Pennsylvania 17033-0850, USA
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the final weighted mean AR CAG repeat length was the average of these two determinations. Antinuclear antibody assay Levels of ANAs were measured in serum samples using the ELISA technique (Inova Diagnostics, San Diego, CA, USA). A positive result in
Nadia Sawicka-Gutaj
Nadia Sawicka-Gutaj
Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland
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Ariadna Zybek-Kocik
Ariadna Zybek-Kocik
Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland
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Michał Kloska
Michał Kloska
Lehigh Valley Health Network, Department of Medicine, Lehigh Valley Hospital – Cedar Crest, Allentown, USA
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Paulina Ziółkowska
Paulina Ziółkowska
Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland
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Agata Czarnywojtek
Agata Czarnywojtek
Department of Pharmacology, Poznan University of Medical Sciences, Poznan, Poland
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Jerzy Sowiński
Jerzy Sowiński
Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland
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Dorota Mańkowska-Wierzbicka
Dorota Mańkowska-Wierzbicka
Department of Gastroenterology, Internal Medicine, Metabolic Diseases and Dietetics, Poznan University of Medical Sciences, Poznan, Poland
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Marek Ruchała
Marek Ruchała
Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland
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after restoration of thyroid function. Thyrotropin receptor antibodies (TRAbs) were assessed in the hyperthyroid group before the onset of therapy and when the euthyroidism was anticipated. ELISA Assay Kit from Phoenix Pharmaceuticals was used to assess
David C Llewellyn
David C Llewellyn
Department of Endocrinology ASO/EASO COM, King’s College Hospital NHS Foundation Trust, Denmark Hill, London, UK
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Rajaventhan Srirajaskanthan
Rajaventhan Srirajaskanthan
Neuroendocrine Tumour Unit, Kings Health Partners ENETS Centre of Excellence, Denmark Hill, London, UK
Faculty of Life Sciences and Medicine, Kings College London, London, UK
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Faculty of Life Sciences and Medicine, Kings College London, London, UK
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Royce P Vincent
Royce P Vincent
Faculty of Life Sciences and Medicine, Kings College London, London, UK
Department of Clinical Biochemistry, King’s College Hospital NHS Foundation Trust, Denmark Hill, London, UK
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Department of Clinical Biochemistry, King’s College Hospital NHS Foundation Trust, Denmark Hill, London, UK
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Catherine Guy
Catherine Guy
Department of Cellular Pathology, Royal Sussex County Hospital, Eastern Road, Brighton, UK
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Eftychia E Drakou
Eftychia E Drakou
Department of Clinical Oncology, Guy’s Cancer Centre – Guy’s and St Thomas’ NHS Foundation Trust, Great Maze Pond, London, UK
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Simon J B Aylwin
Simon J B Aylwin
Department of Endocrinology ASO/EASO COM, King’s College Hospital NHS Foundation Trust, Denmark Hill, London, UK
Neuroendocrine Tumour Unit, Kings Health Partners ENETS Centre of Excellence, Denmark Hill, London, UK
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Neuroendocrine Tumour Unit, Kings Health Partners ENETS Centre of Excellence, Denmark Hill, London, UK
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Ashley B Grossman
Ashley B Grossman
Oxford Centre for Diabetes, Endocrinology and Metabolism, University of Oxford, Oxford, UK
Barts and the London School of Medicine, Centre for Endocrinology, William Harvey Institute, London, UK
Neuroendocrine Tumour Unit, Royal Free Hospital, London, UK
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Barts and the London School of Medicine, Centre for Endocrinology, William Harvey Institute, London, UK
Neuroendocrine Tumour Unit, Royal Free Hospital, London, UK
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John K Ramage
John K Ramage
Neuroendocrine Tumour Unit, Kings Health Partners ENETS Centre of Excellence, Denmark Hill, London, UK
Faculty of Life Sciences and Medicine, Kings College London, London, UK
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Faculty of Life Sciences and Medicine, Kings College London, London, UK
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Georgios K Dimitriadis
Georgios K Dimitriadis
Department of Endocrinology ASO/EASO COM, King’s College Hospital NHS Foundation Trust, Denmark Hill, London, UK
Faculty of Life Sciences and Medicine, School of Life Course Sciences, Obesity Immunometabolism and Diabetes Group, King’s College London, London, UK
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Faculty of Life Sciences and Medicine, School of Life Course Sciences, Obesity Immunometabolism and Diabetes Group, King’s College London, London, UK
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developed in the late 1980s ( 8 ). In the early 2000s, there was a movement to fluorescent and chemiluminescent tests, which were more accurate. Enzyme-linked immunoassay (ELISA) technique was used, but an issue with ELISA was heterophilic antibody
Xiuzhen Hou
Xiuzhen Hou
Division 1 of Obstetrics, Cangzhou Central Hospital, Cangzhou, Hebei, China
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Junfeng Zhang
Junfeng Zhang
Division 1 of Obstetrics, Cangzhou Central Hospital, Cangzhou, Hebei, China
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Hehong Ma
Hehong Ma
Division 1 of Obstetrics, Cangzhou Central Hospital, Cangzhou, Hebei, China
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Ming Li
Ming Li
Division 1 of Obstetrics, Cangzhou Central Hospital, Cangzhou, Hebei, China
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Pei Wang
Pei Wang
Division 1 of Obstetrics, Cangzhou Central Hospital, Cangzhou, Hebei, China
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was determined with UltraSensitive Mouse Insulin ELISA Kit (EMINS, Thermo Fisher Scientific). A top-loading balance (Fisher Scientific) was used to measure body weight. Liver dissection Six pregnant mice were sacrificed on GD 20, and blood and
Luca Boeri
Luca Boeri
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
Department of Urology, Foundation IRCCS Ca’ Granda – Ospedale Maggiore Policlinico, University of Milan, Milan, Italy
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Department of Urology, Foundation IRCCS Ca’ Granda – Ospedale Maggiore Policlinico, University of Milan, Milan, Italy
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Paolo Capogrosso
Paolo Capogrosso
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
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Walter Cazzaniga
Walter Cazzaniga
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
University Vita-Salute San Raffaele, Milan, Italy
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University Vita-Salute San Raffaele, Milan, Italy
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Edoardo Pozzi
Edoardo Pozzi
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
University Vita-Salute San Raffaele, Milan, Italy
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University Vita-Salute San Raffaele, Milan, Italy
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Luigi Candela
Luigi Candela
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
University Vita-Salute San Raffaele, Milan, Italy
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University Vita-Salute San Raffaele, Milan, Italy
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Federico Belladelli
Federico Belladelli
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
University Vita-Salute San Raffaele, Milan, Italy
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University Vita-Salute San Raffaele, Milan, Italy
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Davide Oreggia
Davide Oreggia
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
University Vita-Salute San Raffaele, Milan, Italy
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University Vita-Salute San Raffaele, Milan, Italy
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Eugenio Ventimiglia
Eugenio Ventimiglia
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
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Nicolò Schifano
Nicolò Schifano
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
University Vita-Salute San Raffaele, Milan, Italy
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University Vita-Salute San Raffaele, Milan, Italy
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Giuseppe Fallara
Giuseppe Fallara
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
University Vita-Salute San Raffaele, Milan, Italy
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University Vita-Salute San Raffaele, Milan, Italy
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Marina Pontillo
Marina Pontillo
Laboratory Medicine Service, IRCCS Ospedale San Raffaele, Milan, Italy
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Costantino Abbate
Costantino Abbate
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
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Emanuele Montanari
Emanuele Montanari
Department of Urology, Foundation IRCCS Ca’ Granda – Ospedale Maggiore Policlinico, University of Milan, Milan, Italy
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Francesco Montorsi
Francesco Montorsi
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
University Vita-Salute San Raffaele, Milan, Italy
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University Vita-Salute San Raffaele, Milan, Italy
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Andrea Salonia
Andrea Salonia
Division of Experimental Oncology/Unit of Urology, URI, IRCCS Ospedale San Raffaele, Milan, Italy
University Vita-Salute San Raffaele, Milan, Italy
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University Vita-Salute San Raffaele, Milan, Italy
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-stimulating hormone (FSH) and luteinizing hormone (LH) were measured using a heterogeneous competitive magnetic separation assay (Bayer Immuno 1 System, Bayer Corp.). Inhibin B (InhB) was measured by an ELISA (Beckman Coulter AMH Gen II ELISA). Total testosterone was
Julia Modesto Vicente
Julia Modesto Vicente
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Brazil
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Junia Carolina Santos-Silva
Junia Carolina Santos-Silva
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Brazil
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Caio Jordão Teixeira
Caio Jordão Teixeira
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Brazil
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Dailson Nogueira de Souza
Dailson Nogueira de Souza
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Brazil
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Jean Franciesco Vettorazzi
Jean Franciesco Vettorazzi
Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, Brazil
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Fabiola Sales Furtuoso
Fabiola Sales Furtuoso
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Brazil
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Isabel Gouveia Adabo
Isabel Gouveia Adabo
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Brazil
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Fabio Takeo Sato
Fabio Takeo Sato
Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, State University of Campinas, Campinas, Brazil
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Marco Aurélio Ramirez Vinolo
Marco Aurélio Ramirez Vinolo
Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, State University of Campinas, Campinas, Brazil
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Everardo Magalhães Carneiro
Everardo Magalhães Carneiro
Department of Structural and Functional Biology, Institute of Biology, State University of Campinas, Campinas, Brazil
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Silvana Bordin
Silvana Bordin
Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil
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Gabriel Forato Anhê
Gabriel Forato Anhê
Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas, Campinas, Brazil
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incubated with a fresh KRB buffer with different glucose concentrations for 1 h at 37°C. The cumulative insulin release over 1 h was quantified in duplicate using commercial ELISA kit (Cat No. EZRMI-13K, Merck Millipore). Immunohistochemistry and
Monika Karczewska-Kupczewska
Monika Karczewska-Kupczewska
Department of Internal Medicine and Metabolic Diseases, Medical University of Białystok, Białystok, Poland
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Agnieszka Nikołajuk
Agnieszka Nikołajuk
Department of Prophylaxis of Metabolic Diseases, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
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Magdalena Stefanowicz
Magdalena Stefanowicz
Department of Metabolic Diseases, Medical University of Białystok, Białystok, Poland
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Natalia Matulewicz
Natalia Matulewicz
Department of Metabolic Diseases, Medical University of Białystok, Białystok, Poland
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Irina Kowalska
Irina Kowalska
Department of Internal Medicine and Metabolic Diseases, Medical University of Białystok, Białystok, Poland
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Marek Strączkowski
Marek Strączkowski
Department of Prophylaxis of Metabolic Diseases, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
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insulin, lipids and high-sensitivity C-reactive protein (hsCRP) were measured as described previously ( 23 ). Total serum chemerin was measured with an ELISA kit (Biovendor, Brno, Czech Republic) with a sensitivity of 0.1 ng/mL and with intra-assay and
