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Myrian Velasco Neuroscience Division, Department of Cognitive Neuroscience, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico

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Rosa Isela Ortiz-Huidobro Neuroscience Division, Department of Cognitive Neuroscience, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico

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Carlos Larqué Department of Embryology and Genetics, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico

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Yuriko Itzel Sánchez-Zamora Neuroscience Division, Department of Cognitive Neuroscience, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico

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José Romo-Yáñez Department of Gynecological and Perinatal Endocrinology, Instituto Nacional de Perinatología ‘Isidro Espinosa de los Reyes’, Mexico City, Mexico

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Marcia Hiriart Neuroscience Division, Department of Cognitive Neuroscience, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico

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heparinized tubes, from 12 h fasted control and HSD rats. The plasma levels of TNFA, IL1B, IL6 and IL4 all from Peprotech were determined by ELISA according to the manufacturer’s instructions. Optical density (OD) was measured after 15 min using an ELISA

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Sylvia Thiele Department of Medicine III, Technische Universität Dresden, Dresden, Germany
Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany

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Anke Hannemann Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Greifswald, Germany

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Maria Winzer Department of Medicine III, Technische Universität Dresden, Dresden, Germany
Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany

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Ulrike Baschant Department of Medicine III, Technische Universität Dresden, Dresden, Germany
Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany

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Heike Weidner Department of Medicine III, Technische Universität Dresden, Dresden, Germany
Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany

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Matthias Nauck Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Greifswald, Germany

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Rajesh V Thakker Academic Endocrine Unit, Radcliffe Department of Medicine University of Oxford, Oxford Centre for Diabetes, Endocrinology, and Metabolism, Churchill Hospital, Oxford, UK

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Martin Bornhäuser Department of Medicine I, Technische Universität Dresden, Dresden, Germany
DFG Research Center and Cluster of Excellence for Regenerative Therapies, Technical University, Dresden, Germany

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Lorenz C Hofbauer Department of Medicine III, Technische Universität Dresden, Dresden, Germany
Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany
DFG Research Center and Cluster of Excellence for Regenerative Therapies, Technical University, Dresden, Germany

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Martina Rauner Department of Medicine III, Technische Universität Dresden, Dresden, Germany
Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany

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, Germany) as well as sclerostin (ALPCO) were measured in the serum of mice using commercially available ELISAs. Sclerostin was measured in the cell culture supernatant of hMSC (Biomedica Medizinprodukte GmbH & Co KG, Vienna, Austria). P1NP, osteocalcin

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Rimesh Pal Department of Endocrinology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

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Sanjay Kumar Bhadada Department of Endocrinology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

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Awesh Singhare Department of Endocrinology, P D Hinduja Hospital and Medical Research Centre, Mumbai, India

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Anil Bhansali Department of Endocrinology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

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Sadishkumar Kamalanathan Department of Endocrinology, Jawaharlal Institute of Post Graduate Medical Education and Research, Pondicherry, India

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Manoj Chadha Department of Endocrinology, P D Hinduja Hospital and Medical Research Centre, Mumbai, India

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Phulrenu Chauhan Department of Endocrinology, P D Hinduja Hospital and Medical Research Centre, Mumbai, India

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Ashwani Sood Department of Nuclear Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India

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Vandana Dhiman Department of Endocrinology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

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Dinesh Chandra Sharma Division of Endocrinology, Rabindranath Tagore Medical College, Udaipur, India

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Uma Nahar Saikia Department of Histopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

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Debajyoti Chatterjee Department of Histopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

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Vikas Agashe Department of Orthopaedics, P D Hinduja Hospital and Medical Research Centre, Mumbai, India

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estimation of serum FGF23 levels, using a two-site enzyme-linked immunosorbent assay (Human FGF23 (C-Term) ELISA, Quidel Immutopics, RR 0-150 RU/mL, the coefficient of variation <10%). Patients with the above biochemical profile and elevated or

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Hans Valdemar López Krabbe Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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Jørgen Holm Petersen Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
Section of Biostatistics, University of Copenhagen, Copenhagen, Denmark

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Louise Laub Asserhøj Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
Department of Fertility, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark

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Trine Holm Johannsen Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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Peter Christiansen Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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Rikke Beck Jensen Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark

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Line Hartvig Cleemann Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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Casper P Hagen Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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Lærke Priskorn Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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Niels Jørgensen Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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Katharina M Main Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark

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Anders Juul Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark

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Lise Aksglaede Department of Growth and Reproduction, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
International Centre for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark

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(ELISA) (Immunotech, Beckman Coulter) until 2015 and by chemiluminescence immunoassay (Access2, Beckman Coulter) from 2015 and onward. Based on an internal validation, the results of the two methods were comparable. The LOD and CV for AMH were 0.14 pmol

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Sarantis Livadas Endocrine Unit, Athens Medical Centre, Athens, Greece

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Christina Bothou Department of Endocrinology, Diabetology and Clinical Nutrition, University Hospital of Zurich, Zurich, Switzerland

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Justyna Kuliczkowska-Płaksej Department of Endocrinology, Diabetology and Isotope Therapy, University of Medicine, Wrocław, Poland

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Ralitsa Robeva Ushate ‘acad. IV. Penchev’, Department of Endocrinology, Faculty of Medicine, Medical University-Sofia, Sofia, Bulgaria

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Andromahi Vryonidou Department of Endocrinology and Diabetes, Hellenic Red Cross Hospital, Athens, Greece

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Jelica Bjekic Macut Department of Endocrinology, UMC Bežanijska Kosa, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

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Ioannis Androulakis Endocrine Unit, Athens Medical Centre, Athens, Greece

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Milica Opalic Clinic of Endocrinology, Diabetes and Metabolic Diseases, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

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Zadalla Mouslech 1st Medical Propedeutic, Department of Internal Medicine, AHEPA University Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece

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Andrej Milewicz Department of Endocrinology, Diabetology and Isotope Therapy, University of Medicine, Wrocław, Poland

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Alessandra Gambineri Department of Medical and Surgical Science-DIMEC Endocrinology Unit, University of Bologna – S. Orsola-Mapighi Hospital, Italy

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Dimitrios Panidis Gynaecological Endocrinology Infirmary of the Second Department of Obstetrics and Gynaecology, Aristotle University of Thessaloniki, Thessaloniki, Greece

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Djuro Macut Clinic of Endocrinology, Diabetes and Metabolic Diseases, Faculty of Medicine, University of Belgrade, Belgrade, Serbia

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Clinical Chemistry System (Siemens Medical Solutions). Insulin was measured using a solid-phase enzyme-amplified sensitivity immunoassay (INS-EASIA; Biosource Technologies, Nivelles, Belgium). Total testosterone was measured by ELISA (testosterone enzyme

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Iben Rix Center for Clinical Metabolic Research, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark
Zealand Pharma A/S, Søborg, Denmark

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Marie L Johansen Department of Medicine, Herlev Hospital, University of Copenhagen, Herlev, Denmark

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Asger Lund Center for Clinical Metabolic Research, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark

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Malte P Suppli Center for Clinical Metabolic Research, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark

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Elizaveta Chabanova Department of Radiology, Herlev Hospital, University of Copenhagen, Herlev, Denmark

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Gerrit van Hall Clinical Metabolomics Core Facility, Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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Jens J Holst Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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Nicolai J Wewer Albrechtsen Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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Caroline Kistorp Department of Endocrinology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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Filip K Knop Center for Clinical Metabolic Research, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark
Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Steno Diabetes Center Copenhagen, Herlev, Denmark

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analyses Blood samples were collected after an overnight fast of a minimum of 8 h and after 30 min of rest. Plasma was stored at −80°C until batch analyses. Plasma glucagon was measured from EDTA plasma using a sandwich ELISA (Mercodia, Uppsala, Sweden

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Yufen Zhao College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Erge Namei College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Bingxue Yang College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Xiangnan Bao Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, PR China
National Center of Technology Innovation for Dairy Industry, Hohhot, PR China

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Wei Sun Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot, PR China

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Gerile Subudeng College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Guifang Cao College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Haijun Li College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, PR China
Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, PR China

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Gui Wang Animal Genetic Breeding and Reproduction Research Center, Hetao College, Bayannur, PR China

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-linked immunosorbent assay After culturing a group of ten COCs for IVM, CCs were separated from oocytes, and both were transferred in 100 µL of 0.1 M HCl. Subsequently, cAMP concentrations in the CCs and oocytes were determined by ELISA using the cAMP ELISA kit

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Cornelia Then Medizinische Klinik und Poliklinik IV, Klinikum der Universität München, LMU München, Munich, Germany
Clinical Cooperation Group Diabetes, LMU München and Helmholtz Zentrum München, Munich, Germany

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Holger Then Freie Waldorfschule Augsburg, Augsburg, Germany

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Andreas Lechner Medizinische Klinik und Poliklinik IV, Klinikum der Universität München, LMU München, Munich, Germany
Clinical Cooperation Group Diabetes, LMU München and Helmholtz Zentrum München, Munich, Germany
German Center for Diabetes Research (DZD), München-Neuherberg, Germany

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Cornelia Huth German Center for Diabetes Research (DZD), München-Neuherberg, Germany
Institute of Epidemiology, Helmholtz Zentrum München – German Research Center for Environmental Health (GmbH), Neuherberg, Germany

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Christa Meisinger Independent Research Group Clinical Epidemiology, Helmholtz Zentrum München – German Research Center for Environmental Health (GmbH), Neuherberg, Germany
Chair of Epidemiology at UNIKAT Augsburg, Ludwig-Maximilians-Universität München, Munich, Germany

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Margit Heier Institute of Epidemiology, Helmholtz Zentrum München – German Research Center for Environmental Health (GmbH), Neuherberg, Germany
KORA Study Center, University Hospital Augsburg, Augsburg, Germany

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Annette Peters German Center for Diabetes Research (DZD), München-Neuherberg, Germany
Institute of Epidemiology, Helmholtz Zentrum München – German Research Center for Environmental Health (GmbH), Neuherberg, Germany
DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany

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Wolfgang Koenig DZHK (German Centre for Cardiovascular Research), partner site Munich Heart Alliance, Munich, Germany
Institute of Epidemiology and Medical Biometry, University of Ulm, Ulm, Germany
Deutsches Herzzentrum München, Technische Universität München, Munich, Germany

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Wolfgang Rathmann Institute of Biometrics and Epidemiology, German Diabetes Center, Leibniz Institute at Heinrich Heine University Düsseldorf, Düsseldorf, Germany

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Christian Herder German Center for Diabetes Research (DZD), München-Neuherberg, Germany
Institute of Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Division of Endocrinology and Diabetology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany

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Michael Roden German Center for Diabetes Research (DZD), München-Neuherberg, Germany
Institute of Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf, Düsseldorf, Germany
Division of Endocrinology and Diabetology, Medical Faculty, Heinrich Heine University Düsseldorf, Düsseldorf, Germany

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Jürgen Scherberich Klinikum München-Harlaching, Teaching Hospital of the Ludwig-Maximilians-Universität, Munich, Germany

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Jochen Seissler Medizinische Klinik und Poliklinik IV, Klinikum der Universität München, LMU München, Munich, Germany
Clinical Cooperation Group Diabetes, LMU München and Helmholtz Zentrum München, Munich, Germany

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previous studies using urinary uromodulin, we measured sUmod with a sensitive and robust ELISA. In contrast to uromodulin of urine origin, which forms various polymers with changing episodes and different antigenic sites ( 31 ), sUmod is a stable antigen

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Shilpa Lingaiah Department of Obstetrics and Gynaecology, PEDEGO Research Unit, Medical Research Centre, University of Oulu and Oulu University Hospital, Oulu, Finland

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Laure Morin-Papunen Department of Obstetrics and Gynaecology, PEDEGO Research Unit, Medical Research Centre, University of Oulu and Oulu University Hospital, Oulu, Finland

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Terhi Piltonen Department of Obstetrics and Gynaecology, PEDEGO Research Unit, Medical Research Centre, University of Oulu and Oulu University Hospital, Oulu, Finland

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Inger Sundström-Poromaa Department of Women’s and Children’s Health, Uppsala University, Uppsala, Sweden

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Elisabet Stener-Victorin Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden

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Juha S Tapanainen Department of Obstetrics and Gynaecology, PEDEGO Research Unit, Medical Research Centre, University of Oulu and Oulu University Hospital, Oulu, Finland
Department of Obstetrics and Gynaecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland

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RBP4 were analysed by enzyme-linked immunosorbent assay according to the manufacturer’s instructions (Quantikine ELISA; R&D Systems). The intra- and inter-assay coefficients of variation were 6.5 and 9% respectively. The metabolic variables

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Katarzyna Wyskida Health Promotion and Obesity Management Unit, Department of Pathophysiology, Medical Faculty of Silesia in Katowice, Medical University of Silesia, Katowice, Poland

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Grzegorz Franik Department of Gynecological Endocrinology, Medical Faculty of Silesia in Katowice, Medical University of Silesia, Katowice, Poland

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Tomasz Wikarek Department of Gynecology, Medical Faculty of Silesia in Katowice, Medical University of Silesia, Katowice, Poland

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Aleksander Owczarek Department of Statistics, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland

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Alham Delroba Health Promotion and Obesity Management Unit, Department of Pathophysiology, Medical Faculty of Silesia in Katowice, Medical University of Silesia, Katowice, Poland

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Jerzy Chudek Pathophysiology Unit, Department of Pathophysiology, Medical Faculty of Silesia in Katowice, Medical University of Silesia, Katowice, Poland

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Jerzy Sikora Department of Perinatology and Gynecology, Medical University of Silesia, Katowice, Poland

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Magdalena Olszanecka-Glinianowicz Health Promotion and Obesity Management Unit, Department of Pathophysiology, Medical Faculty of Silesia in Katowice, Medical University of Silesia, Katowice, Poland

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determined by ELISA (DRG Instruments GmbH, Marburg, Germany). The respective intra- and inter-assay coefficients of variations were 5.5% and 6.1% for FSH, 5.6% and 6.2% for LH, 4.5% and 5.9% for PRL, 4.7% and 7.8% for E2, 3.6% and 7.1% for testosterone, 6

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