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Carla Scaroni Dipartimento di Medicina, U.O.C. Endocrinologia, Università di Padova, Padova, Italy

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Nora M Albiger Dipartimento di Medicina, U.O.C. Endocrinologia, Università di Padova, Padova, Italy

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Serena Palmieri Department of Clinical Sciences and Community Health, University of Milan, Fondazione IRCCS Cà Granda-Ospedale Maggiore Policlinico, Milan, Italy

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Davide Iacuaniello Dipartimento di Medicina Clinica e Chirurgia, Sezione di Endocrinologia, Università Federico II di Napoli, Napoli, Italy

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Chiara Graziadio Department of Experimental Medicine, University La Sapienza, Rome, Italy

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Luca Damiani Department of Medical Sciences, Endocrinology and Internal Medicine Section, University of Ferrara, Ferrara, Italy

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Marialuisa Zilio Dipartimento di Medicina, U.O.C. Endocrinologia, Università di Padova, Padova, Italy

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Antonio Stigliano Department of Clinical and Molecular Medicine, Sant’Andrea Hospital, University La Sapienza, Rome, Italy

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Annamaria Colao Dipartimento di Medicina Clinica e Chirurgia, Sezione di Endocrinologia, Università Federico II di Napoli, Napoli, Italy

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Rosario Pivonello Dipartimento di Medicina Clinica e Chirurgia, Sezione di Endocrinologia, Università Federico II di Napoli, Napoli, Italy

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the Altogether to Beat Cushing’s Syndrome (ABC) study group
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chromatography-tandem mass spectrometry (LC–MS/MS). Antibody-based immunoassays can be affected by cross-reactivity (especially cortisone, which has a structure similar to that of cortisol) and synthetic glucocorticoids ( 9 ). In fact, cortisol is converted into

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Cheryl M Isherwood Section of Chronobiology, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom

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M Denise Robertson Section of Metabolic Medicine, Food and Macronutrients, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom

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Debra J Skene Section of Chronobiology, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom

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Jonathan D Johnston Section of Chronobiology, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom

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metabolites exhibiting time-of-day variation using an untargeted liquid chromatography-mass spectrometry metabolomic approach . Chronobiology International 2012 29 868 – 881 . ( https://doi.org/10.3109/07420528.2012.699122 ) 44 Honma A Revell VL Gunn

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Yueyuan Yang Department of Endocrinology, Renmin Hospital of Wuhan University, Wuhan, China

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Tingting Yu Department of Radiology, Renmin Hospital of Wuhan University, Wuhan, China

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Zhili Niu Department of Clinical Laboratory, Institute of translational medicine, Renmin Hospital of Wuhan University, Wuhan, China

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Ling Gao Department of Endocrinology, Renmin Hospital of Wuhan University, Wuhan, China

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acid (v/v) at a flow rate of 0.5 mL/min. The gradient elution was used in the run, and the gradients were as follows: 0–0.8 min 95% B, 0.8–1.3 min 95–60% B, 1.3–3.3 min 60% B, 3.3–3.8 min 60–95% B, 3.8–5.5min 95% B. Mass spectrometry analyses were

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Jonneke J Hollanders Department of Pediatric Endocrinology, VU University Medical Center, Amsterdam, The Netherlands

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Bibian van der Voorn Department of Pediatric Endocrinology, VU University Medical Center, Amsterdam, The Netherlands

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Noera Kieviet Department of Pediatrics, Psychiatry Obstetrics Pediatrics Expert Center, OLVG West, Amsterdam, The Netherlands

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Koert M Dolman Department of Pediatrics, Psychiatry Obstetrics Pediatrics Expert Center, OLVG West, Amsterdam, The Netherlands

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Yolanda B de Rijke Department of Clinical Chemistry, Erasmus MC, University Medical Center, Rotterdam, The Netherlands

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Erica L T van den Akker Department of Pediatric Endocrinology, Erasmus MC Sophia Children’s Hospital, Rotterdam, The Netherlands

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Joost Rotteveel Department of Pediatric Endocrinology, VU University Medical Center, Amsterdam, The Netherlands

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Adriaan Honig Department of Pediatrics, Psychiatry Obstetrics Pediatrics Expert Center, OLVG West, Amsterdam, The Netherlands
Department of Psychiatry, VU University Medical Center, Amsterdam, The Netherlands

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Martijn J J Finken Department of Pediatric Endocrinology, VU University Medical Center, Amsterdam, The Netherlands

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-grade methanol at 25°C for 18 h. These extracts were subsequently centrifuged and cleaned using solid phase extraction. GC concentrations were quantified by liquid chromatography–tandem mass spectrometry LC–MS/MS (Waters XEVO-TQ-S system, Waters Corporation

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Yusaku Mori Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
Department of Diabetes, Metabolism, and Endocrinology, Showa University School of Medicine, Shinagawa, Tokyo, Japan

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Eunhyoung Ko Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

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Rudolf Furrer Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

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Linda C Qu Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

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Stuart C Wiber Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

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I George Fantus Departments of Medicine and Physiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada
Toronto General Research Institute, University Health Network, Toronto, Ontario, Canada
Division of Endocrinology and Metabolism, Leadership Centre for Diabetes, Mount Sinai Hospital, Toronto, Ontario, Canada

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Mario Thevis Center for Preventive Doping Research and Institute of Biochemistry, German Sport University Cologne, Cologne, Germany

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Alan Medline Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, Ontario, Canada
Department of Pathology, Humber River Regional Hospital, Toronto, Ontario, Canada

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Adria Giacca Departments of Physiology and Medicine, Institute of Medical Science, Banting and Best Diabetes Centre, University of Toronto, Toronto, Ontario, Canada

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analogues via liquid chromatography coupled to ion mobility mass spectrometry ( 31 ). Statistics Data are expressed as mean ±  s.e.m . Sample size was calculated to give a power of 80% to detect a twofold difference in tumour multiplicity based on

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Fahim Ebrahimi Division of Endocrinology, Diabetes and Metabolism, Department of Internal Medicine, University of Basel Hospital, Basel, Switzerland
Department of Clinical Research, University of Basel Hospital, Basel, Switzerland

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Sandrine A Urwyler Division of Endocrinology, Diabetes and Metabolism, Department of Internal Medicine, University of Basel Hospital, Basel, Switzerland
Department of Clinical Research, University of Basel Hospital, Basel, Switzerland

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Philipp Schuetz Department of Clinical Research, University of Basel Hospital, Basel, Switzerland
Division of Endocrinology, Diabetes and Metabolism, University Department of Medicine, Kantonsspital Aarau, Aarau, Switzerland

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Beat Mueller Department of Clinical Research, University of Basel Hospital, Basel, Switzerland
Division of Endocrinology, Diabetes and Metabolism, University Department of Medicine, Kantonsspital Aarau, Aarau, Switzerland

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Luca Bernasconi Department of Laboratory Medicine, Kantonsspital Aarau, Aarau, Switzerland

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Peter Neyer Department of Laboratory Medicine, Kantonsspital Aarau, Aarau, Switzerland

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Marc Y Donath Division of Endocrinology, Diabetes and Metabolism, Department of Internal Medicine, University of Basel Hospital, Basel, Switzerland
Department of Clinical Research, University of Basel Hospital, Basel, Switzerland

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Mirjam Christ-Crain Division of Endocrinology, Diabetes and Metabolism, Department of Internal Medicine, University of Basel Hospital, Basel, Switzerland
Department of Clinical Research, University of Basel Hospital, Basel, Switzerland

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using a commercially available and certified liquid chromatography tandem mass spectrometry (LC-MS/MS) assay (MassChrom Steroids; Chromsystems, Munich, Germany). The analyses were performed using an UltiMate 3000 ultra-high-performance liquid

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Ann-Cathrin Koschker Division of Endocrinology and Diabetology, Department of Internal Medicine I, University Hospital, University of Würzburg, Würzburg, Germany
Comprehensive Heart Failure Center, University & University Hospital Würzburg, Würzburg, Germany

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Bodo Warrings Comprehensive Heart Failure Center, University & University Hospital Würzburg, Würzburg, Germany
Department of Psychiatry, Psychosomatics and Psychotherapy, University Hospital, University of Würzburg, Würzburg, Germany

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Caroline Morbach Comprehensive Heart Failure Center, University & University Hospital Würzburg, Würzburg, Germany
Division of Cardiology, Department of Internal Medicine I, University Hospital, University of Würzburg, Würzburg, Germany

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Florian Seyfried Department of General, Visceral, Transplant, Vascular, and Pediatric Surgery, University Hospital, University of Würzburg, Würzburg, Germany

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Nicole Rickert Department of Radiology, University Hospital, University of Würzburg, Würzburg, Germany

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Pius Jung Division of Pneumology, Department of Internal Medicine I, University Hospital, University of Würzburg, Würzburg, Germany

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Andreas Geier Division of Hepatology, Department of Internal Medicine II, University Hospital, University of Würzburg, Würzburg, Germany

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Ulrich Dischinger Division of Endocrinology and Diabetology, Department of Internal Medicine I, University Hospital, University of Würzburg, Würzburg, Germany

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Maike Krauthausen Department of General Practice, University Hospital, University of Würzburg, Würzburg, Germany

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Martin J Herrmann Department of Psychiatry, Psychosomatics and Psychotherapy, University Hospital, University of Würzburg, Würzburg, Germany

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Christine Stier Division of Endocrinology and Diabetology, Department of Internal Medicine I, University Hospital, University of Würzburg, Würzburg, Germany
Department of General, Visceral, Transplant, Vascular, and Pediatric Surgery, University Hospital, University of Würzburg, Würzburg, Germany

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Stefan Frantz Comprehensive Heart Failure Center, University & University Hospital Würzburg, Würzburg, Germany
Division of Cardiology, Department of Internal Medicine I, University Hospital, University of Würzburg, Würzburg, Germany

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Uwe Malzahn Center for Clinical Trials, University Hospital, University of Würzburg, Würzburg, Germany

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Stefan Störk Comprehensive Heart Failure Center, University & University Hospital Würzburg, Würzburg, Germany
Division of Cardiology, Department of Internal Medicine I, University Hospital, University of Würzburg, Würzburg, Germany

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Martin Fassnacht Division of Endocrinology and Diabetology, Department of Internal Medicine I, University Hospital, University of Würzburg, Würzburg, Germany
Comprehensive Heart Failure Center, University & University Hospital Würzburg, Würzburg, Germany

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the WAS Study Group
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the WAS Study Group

-MS/MS, liquid chromatography mass spectrometry; MWT-B, Mehrfachwortschatz-Test B; PHQ, patient health questionnaire; SF-36, short form health survey 36; SQ, standardized questionnaire; TMT, trail-making-tests; VBM, Voxel-based morphometry; VFT, verbal fluency

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Carina Ankarberg-Lindgren Department of Pediatrics, Institute of Clinical Sciences, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

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Aneta Gawlik Department of Pediatrics and Pediatric Endocrinology, Medical University of Silesia, School of Medicine, Katowice, Poland

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Berit Kriström Department of Clinical Sciences/Pediatrics, Umeå University, Umeå, Sweden

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Laura Mazzanti Pediatric Endocrinology and Rare Disease Unit, Department of Medical and Surgical Science, University of Bologna, Bologna, Italy

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Elisabeth J Ruijgrok Department of Pharmacy, Erasmus Medical Center – Sophia Children’s Hospital, University Medical Center Rotterdam, Rotterdam, the Netherlands

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Theo C J Sas Department of Pediatric Endocrinology, Sophia Children’s Hospital, University Medical Center Rotterdam, Rotterdam, the Netherlands
Diabeter, National Diabetes Care and Research Center, Rotterdam, the Netherlands

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-tandem mass spectrometry with sex- and puberty-specific reference intervals . Journal of Steroid Biochemistry and Molecular Biology 2018 183 116 – 124 . ( https://doi.org/10.1016/j.jsbmb.2018.06.005 ) 10.1016/j.jsbmb.2018.06.005 17 Reginster JY Albert

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Martin Zweifel Department of Medical Oncology, University Hospital Bern, Bern, Switzerland

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Beat Thürlimann Breast Centre St. Gallen, Kantonsspital St. Gallen, St. Gallen, Switzerland

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Salome Riniker Breast Centre St. Gallen, Kantonsspital St. Gallen, St. Gallen, Switzerland

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Patrik Weder Breast Centre St. Gallen, Kantonsspital St. Gallen, St. Gallen, Switzerland

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Roger von Moos Kantonsspital Graubünden, Chur, Switzerland

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Olivia Pagani Istituto Oncologico della Svizzera Italiana, Bellinzona, Switzerland

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Martin Bigler SAKK Coordinating Centre, Bern, Switzerland

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Karin M Rothgiesser SAKK Coordinating Centre, Bern, Switzerland

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Christiane Pilop SAKK Coordinating Centre, Bern, Switzerland

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Hanne Hawle SAKK Coordinating Centre, Bern, Switzerland

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Peter Brauchli SAKK Coordinating Centre, Bern, Switzerland

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Coya Tapia Institute of Pathology, University of Bern, Bern, Switzerland

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Wolfgang Schoenfeld CURADIS GmbH, Erlangen, Germany

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Cristiana Sessa Istituto Oncologico della Svizzera Italiana, Bellinzona, Switzerland

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for the Swiss Group for Clinical Cancer Research (SAKK)
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assessed at baseline, once at week 2 and once at week 3 of treatment. High-pressure liquid chromatography with mass spectrometry (HPLC–MS) was used for the determination of 4-OHT and 4-OHA in human plasma, with testosterone-d3 and exemestane being used as

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Annieke C G van Baar Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, the Netherlands

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Andrei Prodan Department of Vascular Medicine, Academic Medical Center, Amsterdam, the Netherlands

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Camilla D Wahlgren Center for Diabetes Research, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark

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Steen S Poulsen Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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Filip K Knop Center for Diabetes Research, Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark
Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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Albert K Groen Department of Vascular Medicine, Academic Medical Center, Amsterdam, the Netherlands
Department of Laboratory Medicine, University of Groningen, University Medical Center, Groningen, the Netherlands

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Jacques J Bergman Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, the Netherlands

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Max Nieuwdorp Department of Vascular Medicine, Academic Medical Center, Amsterdam, the Netherlands
Department of Internal Medicine, VUMC Free University, Amsterdam, the Netherlands
Wallenberg Laboratory, Sahlgrenska Hospital, University of Gothenburg, Gothenburg, Sweden

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Evgeni Levin Department of Vascular Medicine, Academic Medical Center, Amsterdam, the Netherlands
Horaizon BV, Delft, the Netherlands

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standardized meal. At baseline, plasma metabolites were determined by liquid chromatography–mass spectrometry for a panel of 96 metabolites containing either amines, oxidative stressors or lipids as previously described ( 9 , 12 , 13 , 14 ). Duodenal

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