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chromatography-tandem mass spectrometry (LC–MS/MS). Antibody-based immunoassays can be affected by cross-reactivity (especially cortisone, which has a structure similar to that of cortisol) and synthetic glucocorticoids ( 9 ). In fact, cortisol is converted into
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metabolites exhibiting time-of-day variation using an untargeted liquid chromatography-mass spectrometry metabolomic approach . Chronobiology International 2012 29 868 – 881 . ( https://doi.org/10.3109/07420528.2012.699122 ) 44 Honma A Revell VL Gunn
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acid (v/v) at a flow rate of 0.5 mL/min. The gradient elution was used in the run, and the gradients were as follows: 0–0.8 min 95% B, 0.8–1.3 min 95–60% B, 1.3–3.3 min 60% B, 3.3–3.8 min 60–95% B, 3.8–5.5min 95% B. Mass spectrometry analyses were
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-grade methanol at 25°C for 18 h. These extracts were subsequently centrifuged and cleaned using solid phase extraction. GC concentrations were quantified by liquid chromatography–tandem mass spectrometry LC–MS/MS (Waters XEVO-TQ-S system, Waters Corporation
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Toronto General Research Institute, University Health Network, Toronto, Ontario, Canada
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analogues via liquid chromatography coupled to ion mobility mass spectrometry ( 31 ). Statistics Data are expressed as mean ± s.e.m . Sample size was calculated to give a power of 80% to detect a twofold difference in tumour multiplicity based on
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Department of Clinical Research, University of Basel Hospital, Basel, Switzerland
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using a commercially available and certified liquid chromatography tandem mass spectrometry (LC-MS/MS) assay (MassChrom Steroids; Chromsystems, Munich, Germany). The analyses were performed using an UltiMate 3000 ultra-high-performance liquid
Comprehensive Heart Failure Center, University & University Hospital Würzburg, Würzburg, Germany
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Department of Psychiatry, Psychosomatics and Psychotherapy, University Hospital, University of Würzburg, Würzburg, Germany
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Division of Cardiology, Department of Internal Medicine I, University Hospital, University of Würzburg, Würzburg, Germany
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Department of General, Visceral, Transplant, Vascular, and Pediatric Surgery, University Hospital, University of Würzburg, Würzburg, Germany
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Division of Cardiology, Department of Internal Medicine I, University Hospital, University of Würzburg, Würzburg, Germany
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Comprehensive Heart Failure Center, University & University Hospital Würzburg, Würzburg, Germany
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-MS/MS, liquid chromatography mass spectrometry; MWT-B, Mehrfachwortschatz-Test B; PHQ, patient health questionnaire; SF-36, short form health survey 36; SQ, standardized questionnaire; TMT, trail-making-tests; VBM, Voxel-based morphometry; VFT, verbal fluency
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Diabeter, National Diabetes Care and Research Center, Rotterdam, the Netherlands
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-tandem mass spectrometry with sex- and puberty-specific reference intervals . Journal of Steroid Biochemistry and Molecular Biology 2018 183 116 – 124 . ( https://doi.org/10.1016/j.jsbmb.2018.06.005 ) 10.1016/j.jsbmb.2018.06.005 17 Reginster JY Albert
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assessed at baseline, once at week 2 and once at week 3 of treatment. High-pressure liquid chromatography with mass spectrometry (HPLC–MS) was used for the determination of 4-OHT and 4-OHA in human plasma, with testosterone-d3 and exemestane being used as
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Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Department of Laboratory Medicine, University of Groningen, University Medical Center, Groningen, the Netherlands
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Department of Internal Medicine, VUMC Free University, Amsterdam, the Netherlands
Wallenberg Laboratory, Sahlgrenska Hospital, University of Gothenburg, Gothenburg, Sweden
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Horaizon BV, Delft, the Netherlands
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standardized meal. At baseline, plasma metabolites were determined by liquid chromatography–mass spectrometry for a panel of 96 metabolites containing either amines, oxidative stressors or lipids as previously described ( 9 , 12 , 13 , 14 ). Duodenal