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Marloes L P Langelaan Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven, The Netherlands
Department of Clinical Chemistry and Haematology, Zuyderland Medical Centre, Heerlen, The Netherlands

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Jérôme M H Kisters Department of Internal Medicine, Catharina Hospital Eindhoven, Eindhoven, The Netherlands

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Mirjam M Oosterwerff Department of Internal Medicine, Catharina Hospital Eindhoven, Eindhoven, The Netherlands

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Arjen-Kars Boer Clinical Laboratory, Catharina Hospital Eindhoven, Eindhoven, The Netherlands

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. Restituto et al. found a sensitivity and specificity of less than 35%, based on salivary cortisol measurements by an ELISA ( 6 ). In addition, Marcus-Perlman et al. demonstrated that a single determination of basal salivary cortisol by modified RIA

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-18-0085
Volume/Issue:
Volume 7: Issue 4
Open access
Hyun-Ah Kim Department of Surgery, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

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Jihye Choi Department of Surgery, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

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Chan Sub Park Department of Surgery, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

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Min-Ki Seong Department of Surgery, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

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Sung-Eun Hong Department of Translational Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

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Jae-Sung Kim Division of Basic Radiation Bioscience, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

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In-Chul Park Division of Basic Radiation Bioscience, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

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Jin Kyung Lee KIRAMS Radiation Biobank, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

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Woo Chul Noh Department of Surgery, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

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the ASTRRA trial investigators
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-chemotherapy AMH, oestradiol, inhibin B and other clinical factors were analysed in relation to ovarian function resumption. The level of serum AMH, oestradiol and inhibin B was evaluated using ELISA. AMH assays were performed using the kit from USCN Life Science

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-18-0180
Volume/Issue:
Volume 7: Issue 8
Open access
Nikolaj Rittig Department of Internal Medicine and Endocrinology (MEA) and Medical Research Laboratory, Aarhus University Hospital, Aarhus C, Denmark

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Mads Svart Department of Internal Medicine and Endocrinology (MEA) and Medical Research Laboratory, Aarhus University Hospital, Aarhus C, Denmark

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Niels Jessen Research Laboratory for Biochemical Pathology, Institute for Clinical Medicine, Aarhus University Hospital, Aarhus C, Denmark

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Niels Møller Department of Internal Medicine and Endocrinology (MEA) and Medical Research Laboratory, Aarhus University Hospital, Aarhus C, Denmark

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Holger J Møller Department of Clinical Biochemistry Aarhus University Hospital, Aarhus C, Denmark

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Henning Grønbæk Department of Hepatology and Gastroenterology, Aarhus University Hospital, Aarhus C, Denmark

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analysis Blood samples were stored at −20°C and analysed in the same assay after all participants had completed all trials. Serum concentrations of cortisol (ELISA, DRG Cortisol Enzyme Immunoassay Kit, Germany), glucagon (EMD Millipore’s Glucagon

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0296
Volume/Issue:
Volume 7: Issue 1
Open access
Pamela Stratton Office of the Clinical Director, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, USA

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Neelam Giri Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA

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Sonia Bhala Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA

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Martha M Sklavos Leidos Biomedical Research, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA

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Blanche P Alter Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA

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Sharon A Savage Clinical Genetics Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA

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Ligia A Pinto Leidos Biomedical Research, Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA

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subjects who reported having children were considered fertile. AMH measurement Serum AMH was measured using the sensitive Gen II AMH ELISA from Beckman Coulter, Inc. at FNLCR according to the manufacturer’s protocol. Excellent intra- and inter

Article Type:
Research Article
Collections:
Reproduction
DOI:
https://doi.org/10.1530/EC-23-0510
Volume/Issue:
Volume 13: Issue 9
Open access
Yanfei Chen Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China

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Mei Li Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China

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Binrong Liao Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China

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Jingzi Zhong Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China

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Dan Lan Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China

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(μIU/ml) × fasting blood glucose (mmol/L)/22.5. Irisin levels were measured by ELISA (Human Irisin Elisa Kit, CUSABIO, Wuhan, China), and the minimum detectable irisin level was 0.78 ng/mL. The corresponding intra- and inter-assay coefficients of

Article Type:
Research Article
Collections:
Reproduction
DOI:
https://doi.org/10.1530/EC-22-0028
Volume/Issue:
Volume 11: Issue 4
Open access
Sahar Hossam El Hini Diabetes and Endocrinology Unit, Department of Internal Medicine, Faculty of Medicine, Minia University, Minia, Egypt

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Yehia Zakaria Mahmoud Department of Internal Medicine, Faculty of Medicine, Minia University, Minia, Egypt

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Ahmed Abdelfadel Saedii Department of Clinical Pathology, Faculty of Medicine, Minia University, Minia, Egypt

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Sayed Shehata Mahmoud Department of Cardiology, Faculty of Medicine, Minia University, Minia, Egypt

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Mohamed Ahmed Amin Department of Radiology, Faculty of Medicine, Minia University, Minia, Egypt

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Shereen Riad Mahmoud Diabetes and Endocrinology Unit, Department of Internal Medicine, Faculty of Medicine, Minia University, Minia, Egypt

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Ragaa Abdelshaheed Matta Diabetes and Endocrinology Unit, Department of Internal Medicine, Faculty of Medicine, Minia University, Minia, Egypt

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(BioMerieux, Marcy-I’Etoile, France). Specimens stored at −80°C were used to measure the anti-thyroid peroxidase (anti-TPO) antibodies (ORGENTEC, Mainz, Germany), high sensitive C-reactive protein ((hsCRP) (BIOS microwell ELISA Diagnostic Systems Kit, San

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0398
Volume/Issue:
Volume 10: Issue 12
Open access
Huma Qamar Centre for Global Child Health, Hospital for Sick Children, Toronto, Ontario, Canada
Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, Canada

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Nandita Perumal Centre for Global Child Health, Hospital for Sick Children, Toronto, Ontario, Canada
Department of Epidemiology, Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario, Canada

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Eszter Papp Centre for Global Child Health, Hospital for Sick Children, Toronto, Ontario, Canada

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Alison D Gernand Department of Nutritional Sciences, Pennsylvania State University, University Park, Pennsylvania, USA

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Abdullah Al Mahmud Nutrition and Clinical Services Division, International Centre for Diarrhoeal Disease Research (icddr,b), Dhaka, Bangladesh

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Daniel E Roth Centre for Global Child Health, Hospital for Sick Children, Toronto, Ontario, Canada
Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, Canada
Department of Paediatrics, Hospital for Sick Children and University of Toronto, Toronto, Ontario, Canada

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quantified using intact PTH (iPTH) and whole PTH (wPTH) sandwich ELISA kits (Immutopics 60-3100 and 60-3000, respectively, Athens, OH, USA). The iPTH assay measures concentrations of the whole 84 amino acid PTH peptide and long fragments that are missing the

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0056
Volume/Issue:
Volume 10: Issue 3
Open access
Lian Hollander-Cohen Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel

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Benjamin Böhm Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel

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Krist Hausken Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel

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Berta Levavi-Sivan Department of Animal Sciences, The Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel

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when injected to mature female carps in vivo ( 23 ). Isolation of carp ovarian follicles and steroid ELISA Ovaries were collected immediately upon killing the fish and incubated as described previously ( 19 ). After rinsing, the medium was

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0389
Volume/Issue:
Volume 8: Issue 11
Open access
Yusaku Mori Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Showa University School of Medicine, Shinagawa, Tokyo, Japan

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Hiroyuki Shimizu Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Showa University School of Medicine, Shinagawa, Tokyo, Japan
Maebashi Hirosegawa Clinic, Maebashi, Gunma, Japan

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Hideki Kushima Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Showa University School of Medicine, Shinagawa, Tokyo, Japan

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Tomomi Saito Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Showa University School of Medicine, Shinagawa, Tokyo, Japan

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Munenori Hiromura Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Showa University School of Medicine, Shinagawa, Tokyo, Japan

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Michishige Terasaki Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Showa University School of Medicine, Shinagawa, Tokyo, Japan

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Masakazu Koshibu Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Showa University School of Medicine, Shinagawa, Tokyo, Japan

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Hirokazu Ohtaki Department of Anatomy, Showa University School of Medicine, Shinagawa, Tokyo, Japan

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Tsutomu Hirano Division of Diabetes, Metabolism, and Endocrinology, Department of Internal Medicine, Showa University School of Medicine, Shinagawa, Tokyo, Japan

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higher dose than those used in the present study (the highest dose of 10 μg/kg/day is equal to approximately 1 nmol/kg/day) ( 16 , 17 , 18 ). However, plasma nesfatin-1 levels in rodents, as measured by ELISA, vary widely among studies, possibly due to

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-19-0120
Volume/Issue:
Volume 8: Issue 5
Open access
Vickie Braithwaite Medical Research Council (MRC) Human Nutrition Research, MRC Keneba, Elsie Widdowson Laboratories, Fulbourn Road, Cambridge CB1 9NL, UK

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Kerry S Jones Medical Research Council (MRC) Human Nutrition Research, MRC Keneba, Elsie Widdowson Laboratories, Fulbourn Road, Cambridge CB1 9NL, UK
Medical Research Council (MRC) Human Nutrition Research, MRC Keneba, Elsie Widdowson Laboratories, Fulbourn Road, Cambridge CB1 9NL, UK

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Shima Assar Medical Research Council (MRC) Human Nutrition Research, MRC Keneba, Elsie Widdowson Laboratories, Fulbourn Road, Cambridge CB1 9NL, UK

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Inez Schoenmakers Medical Research Council (MRC) Human Nutrition Research, MRC Keneba, Elsie Widdowson Laboratories, Fulbourn Road, Cambridge CB1 9NL, UK

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Ann Prentice Medical Research Council (MRC) Human Nutrition Research, MRC Keneba, Elsie Widdowson Laboratories, Fulbourn Road, Cambridge CB1 9NL, UK
Medical Research Council (MRC) Human Nutrition Research, MRC Keneba, Elsie Widdowson Laboratories, Fulbourn Road, Cambridge CB1 9NL, UK

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manufacturers' instructions. EDTA-plasma samples were used to determine FGF23 concentration by ELISA (C-FGF23: Immutopics, Inc., and I-FGF23 (22) : Kainos, Tokyo, Japan) and intact PTH concentration by IRMA (Immulite, Siemens Healthcare Diagnostics, Camberley

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-13-0070
Volume/Issue:
Volume 3: Issue 1
Open access