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parathyroid adenoma obtained from the same patient at a later parathyroid surgery was also observed (29) . The same authors found significant overexpression of CDKN1B mRNA compared with CDKN1B -non-mutated parathyroid tumors and normal parathyroid. The
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stimulate carnitine palmitoyltransferase Iα (CPT1A), a rate-limiting β-oxidation enzyme ( 40 ). Accordingly, CPT1A mRNA and enzyme activity in the hyperthyroidism animal livers increase significantly ( 41 ); CPT1A is inhibited in the hypothyroidism mice ( 42
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secretory granules. The isolation and sequence analysis of Cpe cDNA clones from bovine ( 16 , 24 ), rat ( 17 ) and human ( 18 ) libraries show that the multiple forms of CPE are not from different mRNA species, and southern analysis of genomic DNA
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observed might correlate with ESR2 expression in lymphoma cells, we tested the effects of BPA and genistein on the growth of Daudi lymphoma cells which express low ESR2 mRNA compared to Granta-519 cells (and no ESR1 , data not shown) (Supplementary Fig
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were changes of serum kisspeptin and MKRN3 levels during the peripubertal period ( 9 , 10 , 11 , 12 ). It has been demonstrated in mice that the expression of KISS-1 mRNA in the anteroventral periventricular nucleus was induced by the onset of
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Sequence Detection System (Roche) were used for qRT-PCR, and mRNA levels were normalized to β-actin. The sequences of the primers used for qRT-PCR are listed in Supplementary Table 1. Western blotting Cells were immediately washed with ice
Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University, Zhenjiang, China
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Biotechnology, Dalian, China) using random hexamers and oligo dT as primers. The qRT–PCR was performed with SYBR ® Premix Ex Taq™ (TliRNaseH Plus) in a real-time PCR Mx3000PTM System (Genetimes Technology, China). For mRNA detection, the primers were as follows
School of Medicine, Universidad de los Andes, Bogotá, Colombia
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Endocrinology Section, Hospital Universitario Fundación Santa Fe de Bogotá, Bogotá, Colombia
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School of Medicine, Universidad de los Andes, Bogotá, Colombia
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enrichment of mRNA using poly-T oligonucleotides in the cDNA transcription and discarding other types of RNA present in the extracted samples. cDNA synthesis quality was confirmed with conventional PCR amplification of an internal control of gene expression
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-stimulated glucose uptake ( 15 ). In humans, white AT lipolytic rate is positively associated with insulin resistance ( 10 ). However, a defect in lipolysis ( 16 ) and decreased AT HSL and ATGL protein expression and/or activity ( 16 , 17 , 18 ), as well as mRNA
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DZHK (German Centre for Cardiovascular Research), Greifswald, Germany
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Institute and Policlinic for Radiology and Interventional Radiology, University Hospital, Carl-Gustav-Carus University Dresden, Dresden, Germany
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DZHK (German Centre for Cardiovascular Research), Greifswald, Germany
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knowledge, none of the existing studies have performed sex-specific analyses even though evidence exists that CMKLR1 mRNA regulation in chronic liver diseases is influenced by sex ( 31 ). Furthermore, our analyses showed that the subjects’ fasting status had