Iben Rix
Iben Rix
Center for Clinical Metabolic Research, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark
Zealand Pharma A/S, Søborg, Denmark
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Zealand Pharma A/S, Søborg, Denmark
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Marie L Johansen
Marie L Johansen
Department of Medicine, Herlev Hospital, University of Copenhagen, Herlev, Denmark
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Asger Lund
Asger Lund
Center for Clinical Metabolic Research, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark
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Malte P Suppli
Malte P Suppli
Center for Clinical Metabolic Research, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark
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Elizaveta Chabanova
Elizaveta Chabanova
Department of Radiology, Herlev Hospital, University of Copenhagen, Herlev, Denmark
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Gerrit van Hall
Gerrit van Hall
Clinical Metabolomics Core Facility, Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Jens J Holst
Jens J Holst
Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Nicolai J Wewer Albrechtsen
Nicolai J Wewer Albrechtsen
Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Caroline Kistorp
Caroline Kistorp
Department of Endocrinology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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Filip K Knop
Filip K Knop
Center for Clinical Metabolic Research, Gentofte Hospital, University of Copenhagen, Hellerup, Denmark
Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Steno Diabetes Center Copenhagen, Herlev, Denmark
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Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Steno Diabetes Center Copenhagen, Herlev, Denmark
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) ( 23 ) with an intra-assay and inter-assay coefficient of variation (CV) of <10% ( 24 ). Individual amino acids were measured from lithium-heparinized plasma using liquid chromatography–tandem mass spectrometry (LS-MS/MS) (TSQ Quantiva, Thermo Fisher
Christian Trummer
Christian Trummer
Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria
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Stefan Pilz
Stefan Pilz
Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria
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Verena Schwetz
Verena Schwetz
Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria
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Barbara Obermayer-Pietsch
Barbara Obermayer-Pietsch
Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria
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Elisabeth Lerchbaum
Elisabeth Lerchbaum
Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria
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.4 nmol/L (vitamin D n = 50, placebo n = 50) 37 (27–50) years <75 nmol/L; 52 (42–66) nmol/L 18.0 (15.8–21.5) nmol/L TT measured using mass spectrometry 12 weeks 20,000 IU/week (~2,857 IU/day) vs placebo No significant effect 107 (89
R Perchard
R Perchard
Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine & Health, University of Manchester, Manchester, UK
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L Magee
L Magee
Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine & Health, University of Manchester, Manchester, UK
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A Whatmore
A Whatmore
Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine & Health, University of Manchester, Manchester, UK
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F Ivison
F Ivison
Department of Biochemistry, Central Manchester University Hospitals NHS Foundation Trust, Manchester, UK
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P Murray
P Murray
Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine & Health, University of Manchester, Manchester, UK
Department of Paediatric Endocrinology, Royal Manchester Children’s Hospital, Central Manchester Foundation Hospitals NHS Trust, Manchester, UK
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Department of Paediatric Endocrinology, Royal Manchester Children’s Hospital, Central Manchester Foundation Hospitals NHS Trust, Manchester, UK
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A Stevens
A Stevens
Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine & Health, University of Manchester, Manchester, UK
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M Z Mughal
M Z Mughal
Department of Paediatric Endocrinology, Royal Manchester Children’s Hospital, Central Manchester Foundation Hospitals NHS Trust, Manchester, UK
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S Ehtisham
S Ehtisham
Department of Paediatric Endocrinology, Royal Manchester Children’s Hospital, Central Manchester Foundation Hospitals NHS Trust, Manchester, UK
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J Campbell
J Campbell
Department of Paediatric Endocrinology, Royal Manchester Children’s Hospital, Central Manchester Foundation Hospitals NHS Trust, Manchester, UK
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S Ainsworth
S Ainsworth
Department of Paediatric Endocrinology, Royal Manchester Children’s Hospital, Central Manchester Foundation Hospitals NHS Trust, Manchester, UK
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M Marshall
M Marshall
Department of Biochemistry, Central Manchester University Hospitals NHS Foundation Trust, Manchester, UK
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M Bone
M Bone
Department of General Paediatrics, Royal Manchester Children’s Hospital, Central Manchester Foundation Hospitals NHS Trust, Manchester, UK
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I Doughty
I Doughty
Department of General Paediatrics, Royal Manchester Children’s Hospital, Central Manchester Foundation Hospitals NHS Trust, Manchester, UK
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P E Clayton
P E Clayton
Division of Developmental Biology & Medicine, School of Medical Sciences, Faculty of Biology, Medicine & Health, University of Manchester, Manchester, UK
Department of Paediatric Endocrinology, Royal Manchester Children’s Hospital, Central Manchester Foundation Hospitals NHS Trust, Manchester, UK
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Department of Paediatric Endocrinology, Royal Manchester Children’s Hospital, Central Manchester Foundation Hospitals NHS Trust, Manchester, UK
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D (serum 25(OH)D) assay Blood samples were centrifuged at 3000 g for 10 min at 17°C, and aliquots of sera were stored at −80°C. 25(OH)D was assayed using a 25-hydroxy vitamin D mass spectrometry method (Chromsystems) on an AB Sciex 5500 instrument
Matilde Calanchini
Matilde Calanchini
Oxford Centre for Diabetes, Endocrinology & Metabolism, Churchill Hospital, University of Oxford, Oxford, UK
Endocrinology & Metabolism Unit, CTO A. Alesini Hospital ASL Roma 2, Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy
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Endocrinology & Metabolism Unit, CTO A. Alesini Hospital ASL Roma 2, Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy
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Michael Tadman
Michael Tadman
Oxford Centre for Diabetes, Endocrinology & Metabolism, Churchill Hospital, University of Oxford, Oxford, UK
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Jesper Krogh
Jesper Krogh
Oxford Centre for Diabetes, Endocrinology & Metabolism, Churchill Hospital, University of Oxford, Oxford, UK
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Andrea Fabbri
Andrea Fabbri
Endocrinology & Metabolism Unit, CTO A. Alesini Hospital ASL Roma 2, Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy
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Ashley Grossman
Ashley Grossman
Oxford Centre for Diabetes, Endocrinology & Metabolism, Churchill Hospital, University of Oxford, Oxford, UK
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Brian Shine
Brian Shine
Oxford Centre for Diabetes, Endocrinology & Metabolism, Churchill Hospital, University of Oxford, Oxford, UK
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.1258/0004563001899780 ) 13 Miller AG Brown H Degg T Allen K Keevil BG . Measurement of plasma 5-hydroxyindoleacetic acid by liquid chromatography tandem mass spectrometry–comparison with HPLC methodology . Journal of Chromatography: B, Analytical Technologies in
Shilpa Lingaiah
Shilpa Lingaiah
Department of Obstetrics and Gynaecology, PEDEGO Research Unit, Medical Research Centre, University of Oulu and Oulu University Hospital, Oulu, Finland
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Laure Morin-Papunen
Laure Morin-Papunen
Department of Obstetrics and Gynaecology, PEDEGO Research Unit, Medical Research Centre, University of Oulu and Oulu University Hospital, Oulu, Finland
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Terhi Piltonen
Terhi Piltonen
Department of Obstetrics and Gynaecology, PEDEGO Research Unit, Medical Research Centre, University of Oulu and Oulu University Hospital, Oulu, Finland
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Inger Sundström-Poromaa
Inger Sundström-Poromaa
Department of Women’s and Children’s Health, Uppsala University, Uppsala, Sweden
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Elisabet Stener-Victorin
Elisabet Stener-Victorin
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
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Juha S Tapanainen
Juha S Tapanainen
Department of Obstetrics and Gynaecology, PEDEGO Research Unit, Medical Research Centre, University of Oulu and Oulu University Hospital, Oulu, Finland
Department of Obstetrics and Gynaecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland
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Department of Obstetrics and Gynaecology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland
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, 23 , 24 ). Of note, the number of subjects varied between analyses owing to a lack of measurements in some cases. Serum concentrations of testosterone and sex hormone-binding globulin (SHBG) were analysed by means of liquid chromatography-mass
Kathrin R Frey
Kathrin R Frey
Department of Medicine I, Endocrine and Diabetes Unit, University Hospital, University of Würzburg, Würzburg, Germany
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Tina Kienitz
Tina Kienitz
Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany
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Julia Schulz
Julia Schulz
Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany
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Manfred Ventz
Manfred Ventz
Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany
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Kathrin Zopf
Kathrin Zopf
Charité – Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany
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Marcus Quinkler
Marcus Quinkler
Endocrinology in Charlottenburg, Berlin, Germany
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Winterer JC Loriaux DL . Daily cortisol production rate in man determuned by stable isotope dilution/mass spectrometry . Journal of Clinical Endocrinology and Metabolism 1991 72 39 – 45 . ( https://doi.org/10.1210/jcem-72-1-39 ) 10.1210/jcem-72
Jay Toulany
Jay Toulany
College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, Canada
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Sebastian D Parlee
Sebastian D Parlee
Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, Michigan, USA
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Christopher J Sinal
Christopher J Sinal
Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada
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Kathryn Slayter
Kathryn Slayter
Canadian Centre for Vaccinology, IWK Health Centre, Nova Scotia Health Authority and Dalhousie University, Halifax, Nova Scotia, Canada
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Shelly McNeil
Shelly McNeil
Canadian Centre for Vaccinology, IWK Health Centre, Nova Scotia Health Authority and Dalhousie University, Halifax, Nova Scotia, Canada
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Kerry B Goralski
Kerry B Goralski
College of Pharmacy, Dalhousie University, Halifax, Nova Scotia, Canada
Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada
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Department of Pharmacology, Dalhousie University, Halifax, Nova Scotia, Canada
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with extreme caution. Ideally, future clinical studies should include measures of total chemerin or specific chemerin isoforms (using isoform-specific antibodies or mass spectrometry) combined with bioassay measures of CMKLR1 and/or GPR1 activation
Barbara J Boucher
Barbara J Boucher
The Blizard Institute, Queen Mary University of London, London, UK
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Alexandridou A Schorr P Stokes CS Volmer DA . Analysis of vitamin D metabolic markers by mass spectrometry: recent progress regarding the ‘gold standard’ method and integration into clinical practice . Mass Spectrometry Reviews 2021 [epub]. ( https
Kevin C J Yuen
Kevin C J Yuen
Departments of Neuroendocrinology and Neurosurgery, Barrow Neurological Institute, University of Arizona College of Medicine and Creighton School of Medicine, Phoenix, Arizona, United States
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Gudmundur Johannsson
Gudmundur Johannsson
Department of Endocrinology, Sahlgrenska University Hospital and Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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Ken K Y Ho
Ken K Y Ho
The Garvan Institute of Medical Research and the Faculty of Medicine, University of New South Wales, Sydney, Australia
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Bradley S Miller
Bradley S Miller
Pediatric Endocrinology, University of Minnesota Medical School, M Health Fairview Masonic Children’s Hospital, Minneapolis, Minnesota, United States
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Ignacio Bergada
Ignacio Bergada
Centro de Investigaciones Endocrinológicas "Dr César Bergadá" (CEDIE), CONICET-FEI-División de Endocrinología, Hospital de Niños Ricardo Gutiérrez, Buenos Aires, Argentina
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Alan D Rogol
Alan D Rogol
Pediatric Diabetes and Endocrinology, University of Virginia, Charlottesville, Virginia, United States
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, immunoassays, and mass spectrometry ( 102 ), and currently, immunoassays are used most frequently. However, diagnostic errors may still be introduced by the large inter-assay variability of commercial and ‘in-house’ GH assays. A study comparing 96 laboratories
Jan Roar Mellembakken
Jan Roar Mellembakken
Division of Gynecology and Obstetrics, Department of Reproductive Medicine, Oslo University Hospital, Oslo, Norway
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Azita Mahmoudan
Azita Mahmoudan
Division of Gynecology and Obstetrics, Department of Reproductive Medicine, Oslo University Hospital, Oslo, Norway
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Lars Mørkrid
Lars Mørkrid
Department of Medical Biochemistry, Oslo University Hospital, Rikshospitalet, Oslo, Norway
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Inger Sundström-Poromaa
Inger Sundström-Poromaa
Department of Women’s and Children’s Health, Uppsala University, Uppsala, Sweden
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Laure Morin-Papunen
Laure Morin-Papunen
Department of Obstetrics and Gynecology, University Hospital of Oulu, University of Oulu, Medical Research Centre Oulu and PEDEGO Research Unit, Oulu, Finland
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Juha S Tapanainen
Juha S Tapanainen
Department of Obstetrics and Gynecology, University Hospital of Oulu, University of Oulu, Medical Research Centre Oulu and PEDEGO Research Unit, Oulu, Finland
Department of Obstetrics and Gynecology, Helsinki University Hospital and University of Helsinki, Helsinki, Uusimaa, Finland
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Department of Obstetrics and Gynecology, Helsinki University Hospital and University of Helsinki, Helsinki, Uusimaa, Finland
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Terhi T Piltonen
Terhi T Piltonen
Department of Obstetrics and Gynecology, University Hospital of Oulu, University of Oulu, Medical Research Centre Oulu and PEDEGO Research Unit, Oulu, Finland
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Angelica Lindén Hirschberg
Angelica Lindén Hirschberg
Department of Women’s and Children’s Health, Karolinska Institutet and Department of Gynecology and Reproductive Medicine, Stockholm, Sweden
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Elisabet Stener-Victorin
Elisabet Stener-Victorin
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
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Eszter Vanky
Eszter Vanky
Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, UK
Department of Gynecology and Obstetrics, St. Olav’s Hospital, Trondheim, Norway
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Department of Gynecology and Obstetrics, St. Olav’s Hospital, Trondheim, Norway
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Pernille Ravn
Pernille Ravn
Department of Gynecology and Obstetrics, Odense University Hospital, Odense, Denmark
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Richard Christian Jensen
Richard Christian Jensen
Department of Endocrinology, Odense University Hospital, Odense, Denmark
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Marianne Skovsager Andersen
Marianne Skovsager Andersen
Department of Endocrinology, Odense University Hospital, Odense, Denmark
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Dorte Glintborg
Dorte Glintborg
Department of Endocrinology, Odense University Hospital, Odense, Denmark
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measured by liquid chromatography–mass spectrometry (LC/MS), and SHBG was measured by chemiluminometric immunoassays as reported earlier ( 11 , 17 ). In Danish women, serum testosterone was analyzed using a specific radio-immunoassay after ether extraction
