Division of General Medicine, Istituto Auxologico Italiano, IRCCS, S. Giuseppe Hospital, Piancavallo di Oggebbio (VB), Italy
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Division of General Medicine, Istituto Auxologico Italiano, IRCCS, S. Giuseppe Hospital, Piancavallo di Oggebbio (VB), Italy
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Division of Endocrinology, Diabetology and Metabolism, Department of Medical Sciences, University of Turin, Turin, Italy
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assays, procedures were performed in accordance with the manufacturers’ instruction and the samples were analysed in duplicate. Serum leptin levels were assessed using a commercially available human ELISA kit (Mediagnost, Reutlinger, Germany). Intra
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.8%, respectively. PAI-1 ELISA PAI-1 levels in the supernatants of JEG-3 and HTR-8/SVneo were measured utilizing commercially available ELISA kit (R&D system, DSE100). A standard curve was obtained in parallel to each assay and results converted into ng
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Hunan Key Laboratory of Organ Fibrosis, Central South University, Changsha, Hunan, China
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Hunan Key Laboratory of Organ Fibrosis, Central South University, Changsha, Hunan, China
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glucose, HbA1c, total cholesterol (TC), HDL-C, LDL-C, and triacylglycerols (TG). For insulin, ghrelin, and LEAP2 measurement, separate blood samples were collected without anticoagulation to obtain serum for ELISA assay. All blood samples were centrifuged
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transporter-8 (aZnT8), and tyrosine phosphatase (aIA2) were detected by RIA- and ELISA-based commercial assays: 21-Hydroxylase Autoantibody ELISA Kit (RSR Ltd. Cardiff, UK), Anti-TPOn RIA kit, ThermoScientific (BRAHMS GmbH, Hennigsdorf, Germany), Anti-TGn RIA
Department of Diabetes & Endocrinology, Skåne University Hospital, Malmö, Sweden
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Department of Diabetes & Endocrinology, Skåne University Hospital, Malmö, Sweden
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Department of Ophthalmology, Skåne University Hospital, Malmö, Sweden
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Department of Diabetes & Endocrinology, Skåne University Hospital, Malmö, Sweden
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-linked immunosorbent assay Cell culture supernatants were harvested, and the protein levels of PGE 2 , IL-1B, IL-6, and IgG were analyzed with commercially available ELISA kits (R & D Systems) according to the manufacturer’s guidelines. Cell culture for flow
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aspiration. AMH assay Blood was withdrawn via an intravenous catheter and transferred into tubes for centrifugation. All serum was isolated from blood samples and was measured using an ELISA kit according to the manufacturer's instructions (AMH ELISA
Adelaide Medical School, The University of Adelaide, Adelaide, Australia
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Adelaide Medical School, The University of Adelaide, Adelaide, Australia
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ELISA as described and validated previously ( 27 ). Within- and between-assay coefficients of variation were 2.3% and 14.7% ( n = 12 plates) and all samples from a single mouse (serial samples for analysis of secretion pattern) were analysed on the same
Medical Research Laboratories, Department of Endocrinology and Diabetes, Department of Pediatrics, Department of Endocrinology and Diabetes, Institute of Clinical Medicine, Aarhus University, Norrebrogade 44, DK-8000 Aarhus C, Denmark
Medical Research Laboratories, Department of Endocrinology and Diabetes, Department of Pediatrics, Department of Endocrinology and Diabetes, Institute of Clinical Medicine, Aarhus University, Norrebrogade 44, DK-8000 Aarhus C, Denmark
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Medical Research Laboratories, Department of Endocrinology and Diabetes, Department of Pediatrics, Department of Endocrinology and Diabetes, Institute of Clinical Medicine, Aarhus University, Norrebrogade 44, DK-8000 Aarhus C, Denmark
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Medical Research Laboratories, Department of Endocrinology and Diabetes, Department of Pediatrics, Department of Endocrinology and Diabetes, Institute of Clinical Medicine, Aarhus University, Norrebrogade 44, DK-8000 Aarhus C, Denmark
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Medical Research Laboratories, Department of Endocrinology and Diabetes, Department of Pediatrics, Department of Endocrinology and Diabetes, Institute of Clinical Medicine, Aarhus University, Norrebrogade 44, DK-8000 Aarhus C, Denmark
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levels were measured by an ELISA (EIA) from Alpco Diagnostics (Salem, NH, USA) with an intra-assay coefficient of variation (CV) of 5% and interassay CV of 9.7–9.8%. Plasma glucose was analyzed in duplicate using the glucose oxidase method (Beckman
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Department of Gynecology and Obstetrics, Division of Genetic Epidemiology, Vitateq Biotechnology GmbH, University of Duisburg-Essen, D-45122 Essen, Germany
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-antibody sandwich ELISA using an affinity-purified biotinylated polyclonal anti-afamin antibody for coating 96-well streptavidin-bound microtiter plates and peroxidase-conjugated MAB N13 for detection (MicroCoat Biotechnologie GmbH, Bernried, Germany). Secondary
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Vascular Research Group, School of Community Based Medicine, Centre for Integrated Genomic Medical Research, Cardiovascular Research Group, Endocrinology and Diabetes, Salford R&D, Department of Endocrinology and Diabetes, Faculty of Medical, Human and Life Sciences, The University of Manchester, Manchester M13 9PT, UK
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Vascular Research Group, School of Community Based Medicine, Centre for Integrated Genomic Medical Research, Cardiovascular Research Group, Endocrinology and Diabetes, Salford R&D, Department of Endocrinology and Diabetes, Faculty of Medical, Human and Life Sciences, The University of Manchester, Manchester M13 9PT, UK
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-assay coefficients of variation (CV) of <4.5 and <8.4% respectively. IGF2 was measured using an ELISA developed using antibodies that have been previously reported (11) . The analytical sensitivity of the assay was <10 ng/ml. The intra-assay and inter