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Anru Wang Department of Pediatrics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Department of Pediatrics, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China

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Xueqin Yan Department of Pediatrics, Boai Hospital of Zhongshan, Zhongshan, China

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Cai Zhang Department of Pediatrics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

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Caiqi Du Department of Pediatrics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

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Wenjun Long Department of Pediatrics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

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Di Zhan Department of Pediatrics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

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Xiaoping Luo Department of Pediatrics, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

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. Fasting serum samples previously preserved in outpatient visits were collected to determined FGF1 levels. Serum FGF1 levels were determined through a sandwich enzyme-linked immunosorbent assay (ELISA; Cloud-Clone Corp., Wuhan, China). Detection range is

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-18-0141
Volume/Issue:
Volume 7: Issue 8
Open access
Emanuela Zaharieva Department of Internal Medicine, University Hospital Alexandrovska, Clinic of Endocrinology, Faculty of Medicine, Medical University-Sofia, Sofia, Bulgaria

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Zdravko Kamenov Department of Internal Medicine, University Hospital Alexandrovska, Clinic of Endocrinology, Faculty of Medicine, Medical University-Sofia, Sofia, Bulgaria

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Tsvetelina Velikova Department of Clinical Immunology, University Hospital St. Ivan Rilski, Laboratory of Clinical Immunology, Faculty of Medicine, Medical University-Sofia, Sofia, Bulgaria

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Adelina Tsakova Department of Clinical Laboratory, University Hospital Alexandrovska, Central Clinical Laboratory, Faculty of Medicine, Medical University-Sofia, Sofia, Bulgaria

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Yosif El-Darawish Laboratory of Tumor Immunology and Cell Therapy, Hyogo College of Medicine, Hyogo, Japan

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Haruki Okamura Laboratory of Tumor Immunology and Cell Therapy, Hyogo College of Medicine, Hyogo, Japan

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detection limit of 1.5 pg/mL and 0.15 mg/L, respectively. IL-18 was analyzed by enzyme-linked immunosorbent assay (ELISA) (Medical & Biological Laboratories Co., Ltd, Nagoya, Japan) with a detection limit of 12.5 pg/mL. GAD65A were assayed by ELISA

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0273
Volume/Issue:
Volume 7: Issue 1
Open access
Xiaolei Hu Department of Endocrinology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, China
Department of Endocrinology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

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Fengling Chen Department of Endocrinology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

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( 22 , 23 , 24 , 26 , 27 ). However, other studies found no correlations between Ig presence and allergic reactions ( 28 , 29 , 30 ). The two classic techniques used to measure IAs are enzyme-linked immunosorbent assay (ELISA) and radioligand

Article Type:
Review Article
DOI:
https://doi.org/10.1530/EC-17-0309
Volume/Issue:
Volume 7: Issue 1
Open access
Shin-ya Ueda Department of Acupuncture, Morinomiya University of Medical Sciences, Osaka, Japan

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Hidehiro Nakahara Department of Acupuncture, Morinomiya University of Medical Sciences, Osaka, Japan

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Eriko Kawai Department of Environmental Physiology for Exercise, Osaka City University Graduate School of Medicine, Osaka, Japan

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Tatsuya Usui Department of Elementary and Preschool Education, Osaka Seikei College, Osaka, Japan

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Shintaro Tsuji Department of Elementary and Preschool Education, Osaka Seikei College, Osaka, Japan

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Tadayoshi Miyamoto Department of Acupuncture, Morinomiya University of Medical Sciences, Osaka, Japan

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, Yanaihara Institute Inc., Shizuoka, Japan), PYY (Human PYY EIA kit, Yanaihara Institute Inc.) and AG (Active Ghrelin ELISA kit, Mitsubishi Kagaku Iatron Inc., Tokyo, Japan) concentrations were assessed by ELISA. ELISA for PYY quantified the total amount of

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0323
Volume/Issue:
Volume 7: Issue 1
Open access
Rafaella Sales de Freitas Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil

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Thiago F A França Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil

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Sabine Pompeia Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil

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(ELISA) for human samples following the supplier's guidelines, in uniplicate (KIT Cloud-Clone Corp. (CCC, Wuhan, China)). The kit used antibodies produced against the full human KISS1 protein, minus the signal peptide. In other words, it used the sequence

Article Type:
Research Article
Collections:
Reproduction
DOI:
https://doi.org/10.1530/EC-22-0165
Volume/Issue:
Volume 11: Issue 10
Open access
Melody Lok-Yi Chan Department of Medicine, University of Hong Kong, Hong Kong SAR

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Sammy Wing-Ming Shiu Department of Medicine, University of Hong Kong, Hong Kong SAR

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Ching-Lung Cheung Department of Pharmacology and Pharmacy, University of Hong Kong, Hong Kong SAR

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Anskar Yu-Hung Leung Department of Medicine, University of Hong Kong, Hong Kong SAR

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Kathryn Choon-Beng Tan Department of Medicine, University of Hong Kong, Hong Kong SAR

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enzymatically on an analyzer (Hitachi 912; Roche Diagnostics), and LDL cholesterol was calculated by the Friedewald equation. Serum levels of IDOL and PCSK9 were measured using commercially available ELISA kits (CUSABIO® Biotech Co., MD, USA; and Quantikine, R

Article Type:
Research Article
Collections:
Cardiovascular
DOI:
https://doi.org/10.1530/EC-22-0019
Volume/Issue:
Volume 11: Issue 6
Open access
Jairo Arturo Pinzón-Cortés Biological Sciences Department, Laboratory of Human Genetics, Universidad de los Andes, Bogotá, Colombia
School of Medicine, Universidad de los Andes, Bogotá, Colombia

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Angelina Perna-Chaux Biological Sciences Department, Laboratory of Human Genetics, Universidad de los Andes, Bogotá, Colombia

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Nicolás Steven Rojas-Villamizar Biological Sciences Department, Laboratory of Human Genetics, Universidad de los Andes, Bogotá, Colombia

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Angélica Díaz-Basabe Biological Sciences Department, Laboratory of Human Genetics, Universidad de los Andes, Bogotá, Colombia

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Diana Carolina Polanía-Villanueva Biological Sciences Department, Laboratory of Human Genetics, Universidad de los Andes, Bogotá, Colombia

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María Fernanda Jácome Biological Sciences Department, Laboratory of Human Genetics, Universidad de los Andes, Bogotá, Colombia

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Carlos Olimpo Mendivil School of Medicine, Universidad de los Andes, Bogotá, Colombia
Endocrinology Section, Hospital Universitario Fundación Santa Fe de Bogotá, Bogotá, Colombia

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Helena Groot Biological Sciences Department, Laboratory of Human Genetics, Universidad de los Andes, Bogotá, Colombia
School of Medicine, Universidad de los Andes, Bogotá, Colombia

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Valeriano López-Segura Biological Sciences Department, Laboratory of Human Genetics, Universidad de los Andes, Bogotá, Colombia
School of Medicine, Universidad de los Andes, Bogotá, Colombia

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.05, ** P  < 0.01, and *** P  < 0.001. The method used for 5mC and 5hmC estimation was ELISA ab117129 and ab117131, respectively. Interestingly, despite the strong difference, when analyzing the methylation levels vs glycemic control in poorly

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-17-0199
Volume/Issue:
Volume 6: Issue 8
Open access
C L Bodinham
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L Smith
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E L Thomas Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

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J D Bell Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

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J R Swann Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

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A Costabile Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

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D Russell-Jones
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A M Umpleby
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M D Robertson
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glucose oxidase method on the YSI 2300 STAT Plus (YSI Life Sciences) with an inter-assay coefficient of variation (CV) of 1.7%. Plasma insulin concentrations during the clamp and MTT were measured by ELISA (Millipore, Billerica, MA, USA) with inter

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-14-0036
Volume/Issue:
Volume 3: Issue 2
Open access
Ping Gu Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China

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Yuege Lin Department of Pathology, Nanjing Medical University, Nanjing, China

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Qi Wan Department of Neurology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China

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Dongming Su Department of Pathology, Nanjing Medical University, Nanjing, China

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Qun Shu Department of Obstetrics, Shanghai First Maternity and Infant Health Hospital, School of Medicine, Tongji University, Shanghai, China

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commercial ELISA kits (Abcam). The results in ELISA assays were measured using a microplate reader (Flexstation 3, Molecular Devices, San Jose, CA, USA). For OGTT in pregnant women, 75 g glucose dissolved in water was orally administrated, and plasma glucose

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-21-0043
Volume/Issue:
Volume 10: Issue 7
Open access
Mai Morsi Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, Braunschweig, Germany

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Torben Schulze Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, Braunschweig, Germany

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Eike Früh Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, Braunschweig, Germany

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Dennis Brüning Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, Braunschweig, Germany

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Uwe Panten Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, Braunschweig, Germany

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Ingo Rustenbeck Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universität Braunschweig, Braunschweig, Germany

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fractionated efflux was determined by ELISA according to the manufacturer’s protocol (Mercodia, Uppsala, Sweden). The islet insulin content was measured by sonicating a group of 30 islets in an ice-cooled microtube for 20 s and measuring the insulin

Article Type:
Research Article
DOI:
https://doi.org/10.1530/EC-20-0289
Volume/Issue:
Volume 9: Issue 8
Open access