Department of Pediatrics, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China
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. Fasting serum samples previously preserved in outpatient visits were collected to determined FGF1 levels. Serum FGF1 levels were determined through a sandwich enzyme-linked immunosorbent assay (ELISA; Cloud-Clone Corp., Wuhan, China). Detection range is
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detection limit of 1.5 pg/mL and 0.15 mg/L, respectively. IL-18 was analyzed by enzyme-linked immunosorbent assay (ELISA) (Medical & Biological Laboratories Co., Ltd, Nagoya, Japan) with a detection limit of 12.5 pg/mL. GAD65A were assayed by ELISA
Department of Endocrinology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
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( 22 , 23 , 24 , 26 , 27 ). However, other studies found no correlations between Ig presence and allergic reactions ( 28 , 29 , 30 ). The two classic techniques used to measure IAs are enzyme-linked immunosorbent assay (ELISA) and radioligand
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, Yanaihara Institute Inc., Shizuoka, Japan), PYY (Human PYY EIA kit, Yanaihara Institute Inc.) and AG (Active Ghrelin ELISA kit, Mitsubishi Kagaku Iatron Inc., Tokyo, Japan) concentrations were assessed by ELISA. ELISA for PYY quantified the total amount of
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(ELISA) for human samples following the supplier's guidelines, in uniplicate (KIT Cloud-Clone Corp. (CCC, Wuhan, China)). The kit used antibodies produced against the full human KISS1 protein, minus the signal peptide. In other words, it used the sequence
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enzymatically on an analyzer (Hitachi 912; Roche Diagnostics), and LDL cholesterol was calculated by the Friedewald equation. Serum levels of IDOL and PCSK9 were measured using commercially available ELISA kits (CUSABIO® Biotech Co., MD, USA; and Quantikine, R
School of Medicine, Universidad de los Andes, Bogotá, Colombia
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Endocrinology Section, Hospital Universitario Fundación Santa Fe de Bogotá, Bogotá, Colombia
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School of Medicine, Universidad de los Andes, Bogotá, Colombia
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School of Medicine, Universidad de los Andes, Bogotá, Colombia
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.05, ** P < 0.01, and *** P < 0.001. The method used for 5mC and 5hmC estimation was ELISA ab117129 and ab117131, respectively. Interestingly, despite the strong difference, when analyzing the methylation levels vs glycemic control in poorly
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glucose oxidase method on the YSI 2300 STAT Plus (YSI Life Sciences) with an inter-assay coefficient of variation (CV) of 1.7%. Plasma insulin concentrations during the clamp and MTT were measured by ELISA (Millipore, Billerica, MA, USA) with inter
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commercial ELISA kits (Abcam). The results in ELISA assays were measured using a microplate reader (Flexstation 3, Molecular Devices, San Jose, CA, USA). For OGTT in pregnant women, 75 g glucose dissolved in water was orally administrated, and plasma glucose
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fractionated efflux was determined by ELISA according to the manufacturer’s protocol (Mercodia, Uppsala, Sweden). The islet insulin content was measured by sonicating a group of 30 islets in an ice-cooled microtube for 20 s and measuring the insulin