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may be controlled both by gene transcription rate and by splice factor recruitment to the pre-mRNA during the process of alternative splicing ( 96 , 97 , 98 ). An early observation was that specific inhibition of the ARwt protein in PC cell lines
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normalized to the level of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA, the product of a housekeeping gene. To monitor efficiency, negative control (RT-) was added to each qPCR assay. The 2 −∆Ct method was applied to calculate fold changes in gene
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found between CNR1 -mRNA level and adiponectin expression, its secretion or circulating adiponectin ( 43 ). After CB 1 antagonism in rats, a higher adipose gene expression and serum level of adiponectin was detected. This finding was proposed as a
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) or decreased ( 15 ) chemerin mRNA expression has been reported in white AT in obesity. The main source of circulating chemerin in obesity is also not clear. Both positive ( 9 , 18 ) and negative ( 17 ) correlations between serum chemerin and AT
Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou, People’s Republic of China
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Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, People’s Republic of China
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Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Guangzhou, People’s Republic of China
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: ' Homosapiens ', 'steatosis', and 'NAFLD'. Datasets meeting the following eligibility criteria were included in this study: (1) mRNA expression profiling was performed using liver tissues; (2) datasets contained SS subject and matched HCs; and (3) datasets
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microarray assay. Endometrium collection and mRNA extraction Mouse uterus was longitudinally dissected and scraped using the back of a scalpel into RNA Later on ice. Total RNA was extracted using an RNeasy Mini-kit (Qiagen) according to the
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Institute of Oncology, The Affiliated Hospital of Jiangsu University, Zhenjiang, China
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thyroid follicular cell line Nthy-ori 3-1 using TRIzol reagent (Takara) following the manufacturer’s instructions. One microgram of RNA was reverse transcribed by a Prime-Script RT reagent Kit (Takara) in a final volume of 20 μL. The mRNA levels were
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Center of Innovation in Personalized Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
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Table 3). Individual gene expression was normalized on Tata-binding protein (TBP) using the 2 −ΔΔCt as a relative quantification method. Bulk RNA-Seq library preparation and sequencing RNA libraries were generated using the human mRNA TruSeq
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described earlier. Transcript quantification Quantitative RT-PCR was performed as described previously ( 10 ). Briefly, mRNA was extracted from the epididymal adipose tissues using TRIzol (Invitrogen), and synthesized into cDNA using the Prime Script
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. Insulin signaling was impaired in skeletal muscle of male CG-IUGR To investigate the insulin signaling in skeletal muscle of male CG-IUGR, the protein and mRNA level of insulin receptor (IR)-β and insulin receptor substrate (IRS)-1, two canonical