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Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark
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Department of Clinical Biochemistry, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
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Department of Clinical Biochemistry, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark
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Department of Pediatrics, Copenhagen University Hospital - Herlev & Gentofte, Copenhagen, Denmark
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Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark
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-0564) using a Wallac DBS Puncher (PerkinElme). Cortisol was extracted from the DBS with 200 μL extraction solvent (acetonitrile/water: 80:20) containing the stable isotopically labeled cortisol-( 13 C3) internal standard (30 nmol/L) by vigorously mixing