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C L Bodinham, L Smith, E L Thomas, J D Bell, J R Swann, A Costabile, D Russell-Jones, A M Umpleby, and M D Robertson

insulin resistant groups, into similar observations in a T2DM cohort. Using an integrated, whole-body approach, stable isotope tracers to differentiate between changes in hepatic and peripheral insulin sensitivity of glucose uptake, body composition

Open access

Nicolai Preisler, Pascal Laforêt, Karen Lindhardt Madsen, Edith Husu, Christoffer Rasmus Vissing, Gitte Hedermann, Henrik Galbo, Christopher Lindberg, and John Vissing

during prolonged (i.e. 1 h) moderate intensity exercise, in patients with Pompe disease. We used cycle-ergometer exercise and stable isotope methodology in combination with indirect calorimetry to determine glucose, glycerol, and fatty acid metabolism

Open access

Leanne Hodson and Fredrik Karpe

hepatic DNL to a greater extent than consumption of the same amount of either glucose or fructose alone ( 65 , 66 , 67 , 68 ). Metabolic studies, utilising stable-isotope labelled fructose, have demonstrated that the contribution of ingested fructose to

Open access

Ingeborg Brønstad, Lars Breivik, Paal Methlie, Anette S B Wolff, Eirik Bratland, Ingrid Nermoen, Kristian Løvås, and Eystein S Husebye

molecular weight of wt 21OH (56 kDa) were cut out for trypsin treatment. Stable isotope-labelled synthetic peptides (N-terminal replacement of lysine ( 13 C 6 , 15 N 2 ) or arginine ( 13 C 6 , 15 N 4 ) respectively) were added in constant amount before

Open access

Jesper Krogh, Peter Plomgaard, Ruth Frikke-Schmidt, Sten Velschow, Jesper Johannesen, Linda Maria Hilsted, Malene Schrøder, and Ulla Feldt-Rasmussen

-0564) using a Wallac DBS Puncher (PerkinElme). Cortisol was extracted from the DBS with 200 μL extraction solvent (acetonitrile/water: 80:20) containing the stable isotopically labeled cortisol-( 13 C3) internal standard (30 nmol/L) by vigorously mixing

Open access

Kathrin R Frey, Tina Kienitz, Julia Schulz, Manfred Ventz, Kathrin Zopf, and Marcus Quinkler

Winterer JC Loriaux DL . Daily cortisol production rate in man determuned by stable isotope dilution/mass spectrometry . Journal of Clinical Endocrinology and Metabolism 1991 72 39 – 45 . ( ) 10.1210/jcem-72

Open access

María L Bacigalupo, Verónica G Piazza, Nadia S Cicconi, Pablo Carabias, Andrzej Bartke, Yimin Fang, Ana I Sotelo, Gabriel A Rabinovich, María F Troncoso, and Johanna G Miquet

Stable Isotope Labeling by amino Acids in Cell Culture (SILAC) and Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) quantitative proteomic analysis identifies galectin-1 as a potential biomarker for predicting sorafenib resistance in liver

Open access

Siphiwe N Dlamini, Zané Lombard, Lisa K Micklesfield, Nigel Crowther, Shane A Norris, Tracy Snyman, Andrew A Crawford, Brian R Walker, and Julia H Goedecke

Extraction of steroids from serum All calibrators, internal standards (labelled with stable isotope deuterium; corticoseterone-d8 and cortisol-d4), and controls were purchased from Chromsystems (Grafelfing, Germany). A liquid–liquid extraction technique

Open access

J A Tamblyn, C Jenkinson, D P Larner, M Hewison, and M D Kilby

.01.049 ) 10.1016/j.jchromb.2016.01.049 40 Ogawa S Ooki S Shinoda K Higashi T. Analysis of urinary vitamin D(3) metabolites by liquid chromatography/tandem mass spectrometry with ESI-enhancing and stable isotope-coded derivatization . Analytical and

Open access

Teresa Lam, Mark McLean, Amy Hayden, Anne Poljak, Birinder Cheema, Howard Gurney, Glenn Stone, Neha Bahl, Navneeta Reddy, Haleh Shahidipour, and Vita Birzniece

, coefficients of variation (CVs) for LRa and Lox are 3.5 and 6.1% respectively ( 18 ). Hepatic urea production The rate of urea production was measured by the urea turnover technique using stable isotope methodology with 15 N 2 -Urea as tracer, as