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status and intestinal integrity Two-day weighed dietary assessment was conducted using Gambian food composition tables (26) in the same way as described previously (13) . H. pylori infection was determined using a non-invasive stable isotope urea
Non-communicable Diseases Research Unit, South African Medical Research Council, Cape Town, South Africa
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Non-communicable Diseases Research Unit, South African Medical Research Council, Cape Town, South Africa
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Extraction of steroids from serum All calibrators, internal standards (labelled with stable isotope deuterium; corticoseterone-d8 and cortisol-d4), and controls were purchased from Chromsystems (Grafelfing, Germany). A liquid–liquid extraction technique
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.01.049 ) 10.1016/j.jchromb.2016.01.049 40 Ogawa S Ooki S Shinoda K Higashi T. Analysis of urinary vitamin D(3) metabolites by liquid chromatography/tandem mass spectrometry with ESI-enhancing and stable isotope-coded derivatization . Analytical and
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Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
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diabetes before and after bariatric surgery: 1-year prospective study . BMJ Open Diabetes Research and Care 2020 8 e001076 . ( https://doi.org/10.1136/bmjdrc-2019-001076 ) 25 Bornø A & Hall van G . Quantitative amino acid profiling and stable
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Clinical Research Center for Metabolic Disease, Gansu Province, Lanzhou, Gansu, China
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14,000 g for 15 min. The upper layer was collected and dried under a nitrogen atmosphere. Stable isotope-labeled analogs are spiked into the samples as internal standards for quantitation. Quality control (QC) samples were prepared by mixing equal
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highly complex and there is still much to learn in this area. Some potential approaches for closing these gaps in knowledge in future studies include developing methods (e.g. with stable isotope tracers) to assess dynamic changes in mitochondrial function
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-ACTH-driven mechanisms. By using stable isotopes, elevated cortisol levels could indeed be attributed to suppressed cortisol metabolism, and, only in patients suffering from hyperinflammation, to a moderately increased cortisol production ( 47 ). The attenuated cortisol