Search Results

You are looking at 1 - 2 of 2 items for

  • Author: J D Bell x
Clear All Modify Search
C L Bodinham
Search for other papers by C L Bodinham in
Google Scholar
PubMed
Close
,
L Smith
Search for other papers by L Smith in
Google Scholar
PubMed
Close
,
E L Thomas Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

Search for other papers by E L Thomas in
Google Scholar
PubMed
Close
,
J D Bell Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

Search for other papers by J D Bell in
Google Scholar
PubMed
Close
,
J R Swann Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

Search for other papers by J R Swann in
Google Scholar
PubMed
Close
,
A Costabile Nutrition, Metabolic and Molecular Imaging Group, Department of Food and Nutritional Sciences, Metabolism and Diabetes Research Group, Faculty of Health and Medical Sciences, University of Surrey, Leggett Building, Guildford, Surrey GU2 7WG, UK

Search for other papers by A Costabile in
Google Scholar
PubMed
Close
,
D Russell-Jones
Search for other papers by D Russell-Jones in
Google Scholar
PubMed
Close
,
A M Umpleby
Search for other papers by A M Umpleby in
Google Scholar
PubMed
Close
, and
M D Robertson
Search for other papers by M D Robertson in
Google Scholar
PubMed
Close

Resistant starch (RS) has been shown to beneficially affect insulin sensitivity in healthy individuals and those with metabolic syndrome, but its effects on human type 2 diabetes (T2DM) are unknown. This study aimed to determine the effects of increased RS consumption on insulin sensitivity and glucose control and changes in postprandial metabolites and body fat in T2DM. Seventeen individuals with well-controlled T2DM (HbA1c 46.6±2 mmol/mol) consumed, in a random order, either 40 g of type 2 RS (HAM-RS2) or a placebo, daily for 12 weeks with a 12-week washout period in between. At the end of each intervention period, participants attended for three metabolic investigations: a two-step euglycemic–hyperinsulinemic clamp combined with an infusion of [6,6-2H2] glucose, a meal tolerance test (MTT) with arterio-venous sampling across the forearm, and whole-body imaging. HAM-RS2 resulted in significantly lower postprandial glucose concentrations (P=0.045) and a trend for greater glucose uptake across the forearm muscle (P=0.077); however, there was no effect of HAM-RS2 on hepatic or peripheral insulin sensitivity, or on HbA1c. Fasting non-esterified fatty acid (NEFA) concentrations were significantly lower (P=0.004) and NEFA suppression was greater during the clamp with HAM-RS2 (P=0.001). Fasting triglyceride (TG) concentrations and soleus intramuscular TG concentrations were significantly higher following the consumption of HAM-RS2 (P=0.039 and P=0.027 respectively). Although fasting GLP1 concentrations were significantly lower following HAM-RS2 consumption (P=0.049), postprandial GLP1 excursions during the MTT were significantly greater (P=0.009). HAM-RS2 did not improve tissue insulin sensitivity in well-controlled T2DM, but demonstrated beneficial effects on meal handling, possibly due to higher postprandial GLP1.

Open access
T P McVeigh Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, NUI Galway, Galway, Ireland

Search for other papers by T P McVeigh in
Google Scholar
PubMed
Close
,
R J Mulligan Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, NUI Galway, Galway, Ireland

Search for other papers by R J Mulligan in
Google Scholar
PubMed
Close
,
U M McVeigh Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, NUI Galway, Galway, Ireland

Search for other papers by U M McVeigh in
Google Scholar
PubMed
Close
,
P W Owens Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, NUI Galway, Galway, Ireland

Search for other papers by P W Owens in
Google Scholar
PubMed
Close
,
N Miller Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, NUI Galway, Galway, Ireland

Search for other papers by N Miller in
Google Scholar
PubMed
Close
,
M Bell Department of Endocrinology, School of Medicine, NUI Galway, Galway, Ireland

Search for other papers by M Bell in
Google Scholar
PubMed
Close
,
F Sebag Department of Endocrine Surgery Centre hospitalo-universitaire de La Conception, Assistance Publique Hôpitaux de Marseille, Marseille, France
Aix-Marseille Université, Faculté de Médecine, Marseille, France

Search for other papers by F Sebag in
Google Scholar
PubMed
Close
,
C Guerin Department of Endocrine Surgery Centre hospitalo-universitaire de La Conception, Assistance Publique Hôpitaux de Marseille, Marseille, France
Aix-Marseille Université, Faculté de Médecine, Marseille, France

Search for other papers by C Guerin in
Google Scholar
PubMed
Close
,
D S Quill Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, NUI Galway, Galway, Ireland

Search for other papers by D S Quill in
Google Scholar
PubMed
Close
,
J B Weidhaas David Geffen School of Medicine, University of California, Los Angeles, USA

Search for other papers by J B Weidhaas in
Google Scholar
PubMed
Close
,
M J Kerin Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, NUI Galway, Galway, Ireland

Search for other papers by M J Kerin in
Google Scholar
PubMed
Close
, and
A J Lowery Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, NUI Galway, Galway, Ireland

Search for other papers by A J Lowery in
Google Scholar
PubMed
Close

Introduction

MicroRNAs (miRNAs) are small noncoding RNA molecules that exert post-transcriptional effects on gene expression by binding with cis-regulatory regions in target messenger RNA (mRNA). Polymorphisms in genes encoding miRNAs or in miRNA–mRNA binding sites confer deleterious epigenetic effects on cancer risk. miR-146a has a role in inflammation and may have a role as a tumour suppressor. The polymorphism rs2910164 in the MIR146A gene encoding pre-miR-146a has been implicated in several inflammatory pathologies, including cancers of the breast and thyroid, although evidence for the associations has been conflicting in different populations. We aimed to further investigate the association of this variant with these two cancers in an Irish cohort.

Methods

The study group comprised patients with breast cancer (BC), patients with differentiated thyroid cancer (DTC) and unaffected controls. Germline DNA was extracted from blood or from saliva collected using the DNA Genotek Oragene 575 collection kit, using crystallisation precipitation, and genotyped using TaqMan-based PCR. Data were analysed using SPSS, v22.

Results

The total study group included 1516 participants. This comprised 1386 Irish participants; 724 unaffected individuals (controls), 523 patients with breast cancer (BC), 136 patients with differentiated thyroid cancer (DTC) and three patients with dual primary breast and thyroid cancer. An additional cohort of 130 patients with DTC from the South of France was also genotyped for the variant. The variant was detected with a minor allele frequency (MAF) of 0.19 in controls, 0.22 in BC and 0.27 and 0.26 in DTC cases from Ireland and France, respectively. The variant was not significantly associated with BC (per allele odds ratio = 1.20 (0.98–1.46), P  = 0.07), but was associated with DTC in Irish patients (per allele OR = 1.59 (1.18–2.14), P = 0.002).

Conclusion

The rs2910164 variant in MIR146A is significantly associated with DTC, but is not significantly associated with BC in this cohort.

Open access