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Eric Seidel, Gudrun Walenda, Clemens Messerschmidt, Benedikt Obermayer, Mirko Peitzsch, Paal Wallace, Rohini Bahethi, Taekyeong Yoo, Murim Choi, Petra Schrade, Sebastian Bachmann, Gerhard Liebisch, Graeme Eisenhofer, Dieter Beule, and Ute I Scholl

normalized to total protein quantity in mg, as previously described ( 17 ). Measurement of supernatant steroid hormones Cells were seeded on a 12-well plate. After 48 h, medium was replaced. After 24 h, cell supernatants were removed and quickly

Open access

Paal Methlie, Steinar Hustad, Ralf Kellman, Bjørg Almås, Martina M Erichsen, Eystein S Husebye, and Kristian Løvås

's syndrome (CS) and congenital adrenal hyperplasia, male hypogonadism and female hyperandrogenism. Although immunoassays have largely been used to measure steroid hormones, over the last decade, mass spectrometry (MS) has increasingly been adopted as it

Open access

Silvia Ciancia, Vanessa Dubois, and Martine Cools

are reversible, with sex steroid production and pubertal development resuming once the medication is discontinued. Several strategies are available to suppress pubertal development and/or the effects of endogenous sex steroids in the context of TGD

Open access

Sirazum Choudhury, Tricia Tan, Katharine Lazarus, and Karim Meeran

cortisol leading to steroid exposure at detrimental times in the day and finally, differences in the biological actions of oral synthetic glucocorticoids versus endogenous cortisol. Interrogation of the EU-AIR registry demonstrated a higher mortality of 1

Open access

Jana Ernst, Katharina Gert, Frank Bernhard Kraus, Ulrike Elisabeth Rolle-Kampczyk, Martin Wabitsch, Faramarz Dehghani, and Kristina Schaedlich

an enzyme machinery enabling them for the de novo synthesis of steroid hormones from cholesterol ( 8 ). Androgenic steroids exhibit a positive gradient from blood to adipose tissue with the precursor androstenedione and dehydroepiandrosterone as

Open access

Milène Tetsi Nomigni, Sophie Ouzounian, Alice Benoit, Jacqueline Vadrot, Frédérique Tissier, Sylvie Renouf, Hervé Lefebvre, Sophie Christin-Maitre, and Estelle Louiset

expression pattern of steroidogenic enzymes and determined the steroid secretion profile using cultured oncocytic cells. We could demonstrate that the oncocytic cells co-secreted cortisol and androgens, harboring therefore an intermediate steroidogenic

Open access

Letícia Ribeiro Oliveira, Carlos Alberto Longui, Guilherme Guaragna-Filho, José Luiz Costa, Rafael Lanaro, David Antônio Silva, Maria Izabel Chiamolera, Maricilda Palandi de Mello, André Moreno Morcillo, Andrea Trevas Maciel-Guerra, and Gil Guerra-Junior

Introduction The determination of steroid hormone concentration, which is part of the initial approach in the diagnosis of disorders of sex development (DSD), is one of the challenges in pediatric endocrinology ( 1 , 2 , 3 ). Based on the

Open access

Sophie Howarth, Luca Giovanelli, Catherine Napier, and Simon H Pearce

as type 1 diabetes, autoimmune hypothyroidism and pernicious anaemia. Complex interplay between genetic and environmental factors gives rise to immune-mediated destruction of the steroid-producing cells of the adrenal cortex. As in type 1 diabetes

Open access

Ingeborg Brønstad, Lars Breivik, Paal Methlie, Anette S B Wolff, Eirik Bratland, Ingrid Nermoen, Kristian Løvås, and Eystein S Husebye

Introduction The CYP21A2 gene encodes the enzyme steroid 21-hydroxylase (21OH), which is essential for steroid synthesis in the adrenal cortex. Mutations in CYP21A2 are the main cause of the autosomal recessive disorder congenital adrenal

Open access

Yiyan Wang, Yaoyao Dong, Yinghui Fang, Yao Lv, Qiqi Zhu, Xiaoheng Li, Qingquan Lian, and Ren-Shan Ge

adding 2 mL ice-cold ether into reaction tubes. The tubes were vortexed vigorously for 1 min and the steroids were extracted with ether. Then, the ether layer was transferred into a glass tube and was dried up under nitrogen. The steroids were plotted