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Florian Schederecker, Alexander Cecil, Cornelia Prehn, Jana Nano, Wolfgang Koenig, Jerzy Adamski, Tanja Zeller, Annette Peters, and Barbara Thorand

, missing values in at least one of the cofounding covariates, persons treated with exogenous sexual hormones (anti-estrogens, androgens, anti-androgens, enzyme-inhibitors, gonadotropin-releasing hormone analogs and anabolic steroids). Further, we excluded

Open access

Rajae Talbi and Victor M Navarro

steroids leading to complementary roles in maintaining reproductive success. Kiss1 AVPV/PeN neurons, which are vestigial in males, have been shown to play a major role in driving the preovulatory LH surge in females in response to the positive feedback of

Open access

Frans H H Leenen, Mordecai P Blaustein, and John M Hamlyn

1991, a substance ‘indistinguishable from ouabain’ was identified by mass spectroscopy (MS) as an endogenous cardiotonic steroid (CTS) in human plasma ( 54 ). This discovery was validated by MS and NMR identification of EO in bovine, rodent and human

Open access

Stine A Holmboe, Ravi Jasuja, Brian Lawney, Lærke Priskorn, Niels Joergensen, Allan Linneberg, Tina Kold Jensen, Niels Erik Skakkebæk, Anders Juul, and Anna-Maria Andersson

used a fixed average level of albumin in both the algorithm by Vermeulen et al. and Zakharov et al. Both testosterone and SHBG were measured using immunoassays. Measurements of sex steroids by use of immunoassays have been criticized for being less

Open access

Jonneke J Hollanders, Bibian van der Voorn, Noera Kieviet, Koert M Dolman, Yolanda B de Rijke, Erica L T van den Akker, Joost Rotteveel, Adriaan Honig, and Martijn J J Finken

throughout pregnancy ( 4 ). During early gestation, maternal GCs are the main supply. By the second half of gestation, the fetal adrenal starts producing its own steroids, predominantly sex steroids (which serve as a substrate for the placental production of

Open access

Mélanie Rouleau, Francis Lemire, Michel Déry, Benoît Thériault, Gabriel Dubois, Yves Fradet, Paul Toren, Chantal Guillemette, Louis Lacombe, Laurence Klotz, Fred Saad, Dominique Guérette, and Frédéric Pouliot

(IA) methods. Although liquid chromatography–tandem MS (LC–MS/MS) is recognized as the gold standard method to measure steroid levels ( 18 ), MS requires specialized equipment and highly skilled technicians, which prevents its use in most clinical

Open access

Alessandro Ciresi, Stefano Radellini, Valentina Guarnotta, Maria Grazia Mineo, and Carla Giordano

estrogen receptors are expressed in the pituitary, regulating GH gene expression and reducing somatostatin receptor expression, leading to enhanced GH secretion ( 6 ). Lastly, the role of gender and sex steroids in modulating the clearance of GH must be

Open access

Lian Hollander-Cohen, Benjamin Böhm, Krist Hausken, and Berta Levavi-Sivan

when injected to mature female carps in vivo ( 23 ). Isolation of carp ovarian follicles and steroid ELISA Ovaries were collected immediately upon killing the fish and incubated as described previously ( 19 ). After rinsing, the medium was

Open access

Fernando Aprile-Garcia, María Antunica-Noguerol, Maia Ludmila Budziñski, Ana C Liberman, and Eduardo Arzt

adaptive responses to stressful circumstances. GCs belong to the steroid hormone family, a group of small lipophilic compounds derived from cholesterol, its common precursor. Steroid hormones are generally grouped according to the receptors they bind and

Open access

Martin Wiegand, David J Halsall, Sarah L Cowan, Kevin Taylor, Robert J B Goudie, Jacobus Preller, and Mark Gurnell

sample was reconstituted in 350 μL of steroid-free serum (DRG Instruments GmbH, Marburg, Germany). Extraction efficiency was calculated using a cohort of 15 anonymised COVID-19 negative control subjects (data not shown). Extraction efficiency was shown to